Department of Ophthalmology, Eye Research Institute Maastricht, University Hospital Maastricht, Maastricht, The Netherlands.
Invest Ophthalmol Vis Sci. 2011 Jun 16;52(7):4256-62. doi: 10.1167/iovs.10-6892.
To investigate the association between soluble apoptosis and adhesion molecules and the development of proliferative vitreoretinopathy (PVR) after reattachment surgery for rhegmatogenous retinal detachment (RRD).
A multiplex immunoassay was used to measure soluble Fas (sFas), sFas ligand (sFasL), soluble intercellular adhesion molecule (sICAM)-1, and soluble vascular cell adhesion molecule (sVCAM)-1 levels in 55 subretinal fluid samples collected during scleral buckling surgery for primary RRD. Seventeen patients who developed a redetachment due to postoperative PVR after reattachment surgery (PVR group) were compared with age-, sex-, and storage-time-matched RRD samples from 38 patients with an uncomplicated postoperative course (RRD group). Ten vitreous samples from patients with macular hole and ten vitreous samples from eye bank eyes served as additional controls.
A 2- to 3-fold increase in levels of sFas, sFasL, sICAM-1, and sVCAM-1 was found in the PVR group compared with those of the RRD group (P < 0.05 for all analytes), as well as a 5- to 20-fold increase in the PVR group compared with those of additional control groups (P < 0.001 for all analytes). Significant associations (P < 0.001) were found between sFas and both sICAM-1 (r = 0.84) and sVCAM-1 (r = 0.93) and between sFasL and both sICAM-1 (r = 0.82) and sVCAM-1 (r = 0.85). In addition, sFas, sFasL, and sVCAM-1 were significantly correlated (P < 0.05) with the extent and duration of retinal detachment.
These findings indicate that an increased expression of soluble apoptosis and adhesion molecules at the time of primary retinal detachment surgery is associated with the future development of PVR.
研究可溶性凋亡和黏附分子与孔源性视网膜脱离（RRD）巩膜扣带手术后发生增殖性玻璃体视网膜病变（PVR）的关系。
采用多重免疫分析法检测 55 例接受巩膜扣带手术的原发性 RRD 患者的视网膜下液中可溶性 Fas（sFas）、sFas 配体（sFasL）、可溶性细胞间黏附分子（sICAM）-1 和可溶性血管细胞黏附分子（sVCAM）-1 的水平。将其中 17 例患者在接受巩膜扣带手术后因术后 PVR 而再次脱离（PVR 组）的患者与 38 例术后无并发症的 RRD 患者（RRD 组）年龄、性别和储存时间匹配的 RRD 样本进行比较。10 例黄斑裂孔患者的玻璃体样本和 10 例眼库眼的玻璃体样本作为额外的对照。
与 RRD 组相比，PVR 组的 sFas、sFasL、sICAM-1 和 sVCAM-1 水平分别升高了 2-3 倍（所有分析物 P <0.05），与额外对照组相比，sFas、sFasL、sICAM-1 和 sVCAM-1 水平分别升高了 5-20 倍（所有分析物 P <0.001）。sFas 与 sICAM-1（r = 0.84）和 sVCAM-1（r = 0.93）之间以及 sFasL 与 sICAM-1（r = 0.82）和 sVCAM-1（r = 0.85）之间均存在显著相关性（P <0.001）。此外，sFas、sFasL 和 sVCAM-1 与视网膜脱离的范围和持续时间显著相关（P <0.05）。
这些发现表明，原发性视网膜脱离手术时可溶性凋亡和黏附分子表达增加与 PVR 的发生有关。
