Cohen M D, Chen C M, Wei C I
Department of Food Science and Human Nutrition, University of Florida, Gainesville 32611.
Int J Immunopharmacol. 1989;11(3):285-92. doi: 10.1016/0192-0561(89)90166-5.
We have previously demonstrated that ammonium metavanadate has a broad immunomodulating effect in mice after subchronic exposure. Because host resistance to pathogenic Listeria monocytogenes and in vitro phagocytic activity of harvested peritoneal macrophages (PEM) were strongly affected by vanadate treatment, we investigated the effect of vanadate on the functional role of resident PEM in active listeriosis in the mouse. Vanadium treatment results in altered patterns of clearance of the organism from the peritoneal cavity, liver and spleen. The total in vitro phagocytic uptake of Listeria by PEM was consistently decreased as a function of infection period. Similarly, intracellular killing of Listeria was decreased although the PEM from the vanadate-treated and control mice were more bacteriostatic than bactericidal. Population distributions of Listeria within infected PEM were not affected by host pretreatment with vanadate. Vanadate exposure interferes with both the uptake and ultimate intraphagolysosomal killing of Listeria. These results were expected in light of our previous studies of the effects of vanadate on PEM superoxide production, and hexose monophosphate shunt and glutathione redox cycle activity. The results provide additional information for the development of a mechanism to explain why workers exposed to vanadium-containing dusts have increased susceptibility to bacterial respiratory diseases.
我们之前已经证明，亚慢性暴露后偏钒酸铵在小鼠中具有广泛的免疫调节作用。由于钒酸盐处理对宿主对致病性单核细胞增生李斯特菌的抵抗力以及收获的腹腔巨噬细胞（PEM）的体外吞噬活性有强烈影响，我们研究了钒酸盐对小鼠活动性李斯特菌病中驻留PEM功能作用的影响。钒处理导致从腹腔、肝脏和脾脏清除该生物体的模式发生改变。随着感染时间的延长，PEM对李斯特菌的体外总吞噬摄取持续下降。同样，李斯特菌的细胞内杀伤作用也降低了，尽管来自钒酸盐处理组和对照组小鼠的PEM抑菌作用比杀菌作用更强。感染的PEM内李斯特菌的种群分布不受宿主钒酸盐预处理的影响。钒酸盐暴露会干扰李斯特菌的摄取以及最终在吞噬溶酶体内的杀伤。鉴于我们之前关于钒酸盐对PEM超氧化物产生、磷酸己糖旁路和谷胱甘肽氧化还原循环活性影响的研究，这些结果是可以预期的。这些结果为解释接触含钒粉尘的工人对细菌性呼吸道疾病易感性增加的机制的发展提供了额外信息。
