Department of Internal Medicine, University of Cincinnati, PO Box 670535, Cincinnati, OH 45267, USA.
Int Arch Occup Environ Health. 2011 Jan;84(1):19-27. doi: 10.1007/s00420-010-0529-z. Epub 2010 Mar 25.
Exposure to environmental tobacco smoke (ETS) leads to molecular damage in the form of DNA adducts. While lung cancer risk is higher among African Americans compared to White Americans, a few studies have tested for racial differences in DNA adducts among children exposed to ETS. The purpose of this study was to test whether African American children have higher DNA adducts levels compared to White children adjusted for ETS exposure.
Data and biologic specimens were drawn from an existing cohort of 212 asthmatic children. These subjects participated in a 12-month ETS-reduction trial that employed HEPA air cleaners with active filter cartridges and sham filter cartridges. White blood cell (WBC) DNA was analyzed for DNA adducts using (32)P-postlabeling. We assessed ETS exposure using a validated air nicotine dosimeter. We determined the independent relationship between African American race and DNA adduct levels adjusted for ETS exposure and air cleaner use.
The mean age of the subjects was 8.4 years; 55% were African American. There was no difference in DNA adduct levels between African American and White children (11.8 vs. 11.2 adducts per 10(9) nucleotides, p = 0.86), despite slightly higher levels of air nicotine exposure (3.4 vs. 2.2 μg/m(3), p = 0.14). African American children used their air cleaners less often than White children. We found that the best predictor of DNA adduct levels was the duration of air cleaner use (r = -0.133, p = 0.056). This association was independent of cartridge type.
We did not see differences in adduct levels by race even after accounting for the level of ETS exposure. However, there was a marginal inverse association between air cleaner use and adducts. Additional research is required to understand this phenomenon.
接触环境烟草烟雾（ETS）会导致 DNA 加合物形式的分子损伤。虽然非裔美国人患肺癌的风险高于白种人，但少数研究已经测试了暴露于 ETS 的儿童中 DNA 加合物的种族差异。本研究旨在测试非裔美国儿童的 DNA 加合物水平是否高于调整 ETS 暴露后白种儿童的水平。
数据和生物标本取自 212 名哮喘儿童的现有队列。这些受试者参加了为期 12 个月的 ETS 减少试验，该试验使用带有活性过滤筒和假过滤筒的高效空气净化器。使用（32）P-后标记法分析白细胞（WBC）DNA 中的 DNA 加合物。我们使用经过验证的空气尼古丁剂量计评估 ETS 暴露情况。我们确定了非裔美国种族与调整 ETS 暴露和空气净化器使用后的 DNA 加合物水平之间的独立关系。
受试者的平均年龄为 8.4 岁，其中 55%是非裔美国人。尽管空气尼古丁暴露水平略高（3.4 与 2.2μg/m（3），p=0.14），但非裔美国儿童与白种儿童的 DNA 加合物水平无差异（11.8 与 11.2 个加合物/10（9）个核苷酸，p=0.86）。非裔美国儿童比白种儿童使用空气净化器的频率更低。我们发现，空气净化器使用时间是 DNA 加合物水平的最佳预测因素（r=-0.133，p=0.056）。这种关联独立于筒式类型。
即使考虑到 ETS 暴露水平，我们也没有看到种族之间加合物水平的差异。然而，空气净化器使用与加合物之间存在边缘负相关。需要进一步研究以了解这种现象。
