Institute of Animal Physiology, Slovak Academy of Sciences, Kosice 04001, Slovakia.
Folia Microbiol (Praha). 2010 Jan;55(1):79-82. doi: 10.1007/s12223-010-0013-x. Epub 2010 Mar 25.
The development of resistance to quinolones (nalidixic acid, ciprofloxacin and enrofloxacin) in 2006-2008 was evaluated in 317 strains of Escherichia coli isolated from healthy chicken broilers from various farms. The isolates (2006/2007/2008) showed a high resistance to nalidixic acid (87/85/67 %), ciprofloxacin (CIP) (49/54/29 %) and enrofloxacin (ENR) (52/42/22 %). Nalidixic acid-resistant isolates with low level of MIC for CIP and ENR represented a single mutation; intermediary MIC for CIP and ENR were related to two mutations and high level resistance MIC for CIP (> or =4 mg/L) and ENR (> or =16 mg/L) represented three mutations (two in gyrA and one in parC). There was a correlation between the phenotype reading of high-level resistance and mutations in gyrA (Ser83Leu, Asp87Tyr or Asp87Asn) and parC (Ser80Ile) gene. Plasmid-mediated quinolone-resistance qnrS gene was detected in one Escherichia coli strain with a high level of ciprofloxacin resistance. Our results demonstrate the increase in occurrence of multiresistant E. coli strains with a high level of chromosomal and plasmid resistance to fluoroquinolones.
2006-2008 年，在从不同农场的健康肉鸡中分离出的 317 株大肠杆菌中评估了对喹诺酮类药物（萘啶酸、环丙沙星和恩诺沙星）的耐药性发展情况。这些分离株（2006/2007/2008 年）对萘啶酸（87/85/67%）、环丙沙星（CIP）（49/54/29%）和恩诺沙星（ENR）（52/42/22%）表现出高度耐药性。对 CIP 和 ENR 的 MIC 值较低的萘啶酸耐药分离株只有一个突变；对 CIP 和 ENR 的 MIC 值处于中间水平与两个突变有关，而对 CIP（>或=4mg/L）和 ENR（>或=16mg/L）的高水平耐药性 MIC 则代表三个突变（gyrA 中的两个和 parC 中的一个）。高水平耐药表型与 gyrA（Ser83Leu、Asp87Tyr 或 Asp87Asn）和 parC（Ser80Ile）基因中的突变之间存在相关性。在一株对环丙沙星具有高水平耐药性的大肠杆菌中检测到了质粒介导的喹诺酮耐药 qnrS 基因。我们的研究结果表明，具有高水平染色体和质粒对氟喹诺酮类药物耐药性的多耐药大肠杆菌菌株的发生率增加。
