Veterinary Epidemiology and Economics Research Unit, School of Agriculture, Policy and Development, University of Reading, Reading, Berkshire, UK.
J Appl Microbiol. 2008 Nov;105(5):1421-31. doi: 10.1111/j.1365-2672.2008.03877.x. Epub 2008 Sep 5.
To estimate the proportions of farms on which broilers, turkeys and pigs were shedding fluoroquinolone (FQ)-resistant Escherichia coli or Campylobacter spp. near to slaughter.
Freshly voided faeces were collected on 89 poultry and 108 pig farms and cultured with media containing 1.0 mg l(-1) ciprofloxacin. Studies demonstrated the specificity of this sensitive method, and both poultry and pig sampling yielded FQ-resistant E. coli on 60% of farms. FQ-resistant Campylobacter spp. were found on around 22% of poultry and 75% of pig farms. The majority of resistant isolates of Campylobacter (89%) and E. coli (96%) tested had minimum inhibitory concentrations for ciprofloxacin of > or =8 mg l(-1). The proportion of resistant E. coli and Campylobacter organisms within samples varied widely.
FQ resistance is commonly present among two enteric bacterial genera prevalent on pig and poultry farms, although the low proportion of resistant organisms in many cases requires a sensitive detection technique.
FQ-resistant bacteria with zoonotic potential appear to be present on a high proportion of UK pig and poultry farms. The risk this poses to consumers relative to other causes of FQ-resistant human infections remains to be clarified.
估计临近屠宰时，肉鸡、火鸡和猪的粪便中携带氟喹诺酮（FQ）耐药大肠杆菌或弯曲杆菌属的农场比例。
在 89 个家禽养殖场和 108 个猪圈采集新鲜粪便，并在含有 1.0 mg l(-1)环丙沙星的培养基中进行培养。研究证明了这种敏感方法的特异性，家禽和猪的采样均在 60%的农场中产生了 FQ 耐药大肠杆菌。在约 22%的家禽养殖场和 75%的猪圈中发现了 FQ 耐药弯曲杆菌属。大多数耐药的弯曲杆菌（89%）和大肠杆菌（96%）的分离株对环丙沙星的最小抑菌浓度>或 =8 mg l(-1)。样本中耐药大肠杆菌和弯曲杆菌的比例差异很大。
FQ 耐药性在常见于猪和家禽养殖场的两种肠内细菌属中普遍存在，尽管在许多情况下，耐药菌的比例较低需要一种敏感的检测技术。
具有人畜共患潜力的 FQ 耐药菌似乎存在于英国大量的猪和家禽养殖场中。相对于其他 FQ 耐药性人类感染的原因，这对消费者构成的风险仍需阐明。
