RNA病毒克隆群体的相对适应性和高度适应性的定量分析。
Quantitation of relative fitness and great adaptability of clonal populations of RNA viruses.
作者信息
Holland J J, de la Torre J C, Clarke D K, Duarte E
机构信息
Institute of Molecular Genetics, University of California at San Diego, La Jolla 92093-0116.
出版信息
J Virol. 1991 Jun;65(6):2960-7. doi: 10.1128/JVI.65.6.2960-2967.1991.
Abstract
We describe a sensitive, internally controlled method for comparing the genetic adaptability and relative fitness of virus populations in constant or changing host environments. Certain monoclonal antibody-resistant mutants of vesicular stomatitis virus can compete equally during serial passages in mixtures with the parental wild-type clone from which they were derived. These genetically marked "surrogate wild-type" neutral mutants, when mixed with wild-type virus, allow reliable measurement of changes in virus fitness and of virus adaptation to different host environments. Quantitative fitness vector plots demonstrate graphically that even clones of an RNA virus are composed of complex variant populations (quasispecies). Variants of greater fitness (competitive replication ability) were selected within very few passages of virus clones in new host cells or animals. Even clones which were well adapted to BHK21 cells gained further fitness during repeated passages in BHK21 cells.
摘要
我们描述了一种灵敏的、内部控制的方法，用于比较病毒群体在恒定或变化的宿主环境中的遗传适应性和相对适合度。水泡性口炎病毒的某些单克隆抗体抗性突变体在与它们所衍生的亲本野生型克隆混合进行连续传代时能够同等竞争。这些经基因标记的“替代野生型”中性突变体与野生型病毒混合时，可对病毒适合度的变化以及病毒对不同宿主环境的适应性进行可靠测量。定量适合度向量图以图形方式表明，即使是RNA病毒的克隆也由复杂的变异群体（准种）组成。在新宿主细胞或动物中，病毒克隆经过很少几代传代就会选择出适合度更高（竞争复制能力更强）的变体。即使是非常适应BHK21细胞的克隆，在BHK21细胞中反复传代期间也会获得进一步的适合度。
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本文引用的文献
1
Studies on an attenuated measles-virus vaccine. I. Development and preparations of the vaccine: technics for assay of effects of vaccination.减毒麻疹病毒疫苗的研究。I. 疫苗的研发与制备：疫苗接种效果的检测技术
N Engl J Med. 1960 Jul 28;263:153-9. doi: 10.1056/NEJM196007282630401.
2
Rapid evolution of RNA genomes.RNA基因组的快速进化。
Science. 1982 Mar 26;215(4540):1577-85. doi: 10.1126/science.7041255.
3
Vesicular stomatitis virus mutants resistant to defective-interfering particles accumulate stable 5'-terminal and fewer 3'-terminal mutations in a stepwise manner.对缺陷干扰颗粒具有抗性的水疱性口炎病毒突变体以逐步方式积累稳定的5'-末端和较少的3'-末端突变。
J Virol. 1984 Mar;49(3):793-8. doi: 10.1128/JVI.49.3.793-798.1984.
4
Multiple genetic variants arise in the course of replication of foot-and-mouth disease virus in cell culture.口蹄疫病毒在细胞培养物中复制过程中会出现多种基因变异。
Virology. 1983 Jul 30;128(2):310-8. doi: 10.1016/0042-6822(83)90258-1.
5
High multiplicities of infection favor rapid and random evolution of vesicular stomatitis virus.高感染复数有利于水疱性口炎病毒的快速随机进化。
Virology. 1982 May;119(1):96-108. doi: 10.1016/0042-6822(82)90068-x.
6
Multiple genetic changes can occur in the oral poliovaccines upon replication in humans.
J Gen Virol. 1981 Oct;56(Pt 2):337-47. doi: 10.1099/0022-1317-56-2-337.
7
Nucleotide sequence heterogeneity of the RNA from a natural population of foot-and-mouth-disease virus.口蹄疫病毒自然群体中RNA的核苷酸序列异质性
Gene. 1980 Nov;11(3-4):333-46. doi: 10.1016/0378-1119(80)90073-6.
8
Similar frequencies of antigenic variants in Sendai, vesicular stomatitis, and influenza A viruses.仙台病毒、水疱性口炎病毒和甲型流感病毒中抗原变异体的频率相似。
Virology. 1980 Jul 15;104(1):235-8. doi: 10.1016/0042-6822(80)90382-7.
9
Antigenic determinants of vesicular stomatitis virus: analysis with antigenic variants.水疱性口炎病毒的抗原决定簇：用抗原变异体进行分析
J Immunol. 1983 Jan;130(1):394-8.
10
The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. II. Monoclonal antibodies of nonneutralizing and cross-reactive epitopes of Indiana and New Jersey serotypes.抗体与水疱性口炎病毒主要表面糖蛋白的相互作用。II. 印第安纳血清型和新泽西血清型非中和及交叉反应表位的单克隆抗体
Virology. 1982 Aug;121(1):168-74. doi: 10.1016/0042-6822(82)90126-x.
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