Shanghai Burn Institute, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 197 Ruijin No.2 Road, Shanghai, 200025, People's Republic of China.
Mol Biol Rep. 2011 Jan;38(1):387-94. doi: 10.1007/s11033-010-0120-4. Epub 2010 Mar 26.
The behavior of fibroblasts on patterned substrates was examined in order to elucidate the role of dermal structure in wound healing. Dermal fibroblasts were cultured on micro-patterned silicone elastomer substrates designed to enforce cell adhesion only to fibronectin microdots. The morphology, expression of α-smooth muscle actin (α-SMA), proliferation, apoptotic cells, and soluble collagen production of cells were measured. Cells grown on patterned substrates showed some signs of a scar-fibroblast phenotype such as: elongated pseudopodia, enhanced expression of alpha smooth muscle actin (α-SMA), and increased collagen/pre-collagen, in comparison to unpatterned controls. Cells also showed low proliferation rates and high apoptotic index. The results showed that the microdot arrays, acting as a grid of limited focal adhesion sites, could force cells to adopt constrained morphologies and limited adhesion areas, which affect the cytoskeleton, ultimately leading to expression of a scar-tissue fibroblast phenotype. This study provides insight into the regulatory mechanisms of micro-topology on cell behavior in wound healing.
为了阐明皮肤结构在伤口愈合中的作用，研究了成纤维细胞在图案化基底上的行为。将真皮成纤维细胞培养在微图案化硅酮弹性体基底上，该基底设计用于仅增强对纤维连接蛋白微点的细胞黏附。测量细胞的形态、α-平滑肌肌动蛋白 (α-SMA) 的表达、增殖、凋亡细胞和可溶性胶原的产生。与无图案对照相比，在图案化基底上生长的细胞表现出一些瘢痕成纤维细胞表型的迹象，例如：伸长的伪足、α-平滑肌肌动蛋白 (α-SMA) 的表达增强以及胶原/原胶原增加。细胞还表现出低增殖率和高凋亡指数。结果表明，微点阵列作为有限黏附位点的网格，可以迫使细胞采用受限的形态和有限的黏附区域，这会影响细胞骨架，最终导致表达瘢痕组织成纤维细胞表型。这项研究深入了解了微拓扑结构对伤口愈合中细胞行为的调控机制。
