Department of Conservative Dentistry, University Hospital Jena, Jena, Germany.
J Periodontol. 2010 Jul;81(7):975-81. doi: 10.1902/jop.2010.090246.
The purpose of this study is to clinically and microbiologically evaluate the effect of photodynamic therapy (PDT) as a full-mouth procedure in Fusobacterium nucleatum-infected patients with periodontitis.
In the present study, PDT is administered adjuvantly after scaling and root planing (SRP) to patients with localized chronic periodontitis (LCP). Twenty-four patients, in whom only F. nucleatum was detected by baseline polymerase chain reaction (PCR) after SRP, were randomly assigned to PDT and control groups. PDT was carried out once as a full-mouth disinfection in the test group. The control group was treated with the photosensitizer solution, but without laser irradiation. In all subjects, the clinical parameters plaque index, reddening, bleeding on probing (BOP), probing depth (PD), gingival recession, and clinical attachment level (CAL) were determined at the baseline examination and at 1, 4, and 12 weeks after PDT. Quantitative analysis of the F. nucleatum DNA concentration was performed by competitive PCR. All clinical indices were calculated for each test and control subject as were the median and interquartile range of each group.
In patients with LCP who received PDT treatment, significant reductions in reddening, BOP, and mean PD and CAL were observed during the observation period and with respect to controls. Four and 12 weeks after PDT, the mean PD and CAL showed significant differences from baseline values and from those of the control group. In the PDT group, 12 weeks after treatment, the F. nucleatum DNA concentration was found to be significantly reduced compared to the baseline level.
The results of this study show that the adjuvant application of the described PDT method is appropriate to reduce periodontal inflammatory symptoms and to successfully treat infection with F. nucleatum.
本研究旨在临床和微生物学上评估光动力疗法（PDT）作为伴核梭杆菌感染的牙周炎全口治疗程序的效果。
在本研究中，PDT 作为辅助治疗手段，在牙周炎患者接受龈下刮治和根面平整（SRP）治疗后使用。24 名患者在 SRP 后仅通过基线聚合酶链反应（PCR）检测到核梭杆菌，他们被随机分配到 PDT 组和对照组。PDT 组在试验组中作为全口消毒进行一次治疗。对照组给予光敏剂溶液，但不进行激光照射。所有患者在基线检查时以及 PDT 后 1、4 和 12 周时，记录菌斑指数、红肿、探诊出血（BOP）、探诊深度（PD）、牙龈退缩和临床附着丧失（CAL）等临床参数。通过竞争性 PCR 定量分析核梭杆菌 DNA 浓度。对每个试验和对照受试者计算每个测试和对照受试者的所有临床指标，以及每组的中位数和四分位间距。
在接受 PDT 治疗的 LCP 患者中，在观察期间和与对照组相比，红肿、BOP 和平均 PD 和 CAL 显著降低。PDT 后 4 和 12 周，平均 PD 和 CAL 与基线值和对照组相比有显著差异。在 PDT 组中，治疗 12 周后，核梭杆菌 DNA 浓度与基线水平相比显著降低。
本研究结果表明，该描述性 PDT 方法的辅助应用可以减轻牙周炎症症状，并成功治疗核梭杆菌感染。
