Coburn S P, Ziegler P J, Costill D L, Mahuren J D, Fink W J, Schaltenbrand W E, Pauly T A, Pearson D R, Conn P S, Guilarte T R
Biochemistry Department, Fort Wayne State Developmental Center, IN.
Am J Clin Nutr. 1991 Jun;53(6):1436-42. doi: 10.1093/ajcn/53.6.1436.
Previous reports indicated that in growing rats the vitamin B-6 pool in muscle was relatively stable during deficiency but increased in response to increased vitamin B-6 intake. To determine whether human muscle would show a similar response 10 college-aged males received a low vitamin B-6 diet (1.76 mumol/d) for 6 wk followed by 6 wk on a self-selected diet supplemented with 0.98 mmol pyridoxine HCl/d. During depletion, excretion of pyridoxic acid rapidly adjusted to approximate the intake. Plasma pyridoxal phosphate concentrations at the end of the baseline, depletion, and supplementation periods were 81 +/- 51, 9 +/- 3, and 455 +/- 129 nmol/L, respectively, whereas muscle concentrations were 21 +/- 9, 20 +/- 4, and 25 +/- 7 nmol/g, respectively and total vitamin B-6 in muscle was 28 +/- 10, 27 +/- 4, and 35 +/- 10 nmol/g, respectively. These data provide further confirmation that the vitamin B-6 pools in skeletal muscle are resistant to depletion. They also demonstrate that in humans with constant body weight, vitamin B-6 supplementation is not associated with marked increases in vitamin B-6 in muscle.
先前的报告表明，在生长中的大鼠体内，肌肉中的维生素B-6储备在缺乏期间相对稳定，但会随着维生素B-6摄入量的增加而增加。为了确定人类肌肉是否会有类似反应，10名大学年龄男性先接受低维生素B-6饮食（1.76 μmol/天）6周，随后6周采用自行选择的饮食，并每天补充0.98 mmol盐酸吡哆醇。在消耗期，尿吡哆酸的排泄迅速调整至接近摄入量。基线期、消耗期和补充期结束时，血浆磷酸吡哆醛浓度分别为81±51、9±3和455±129 nmol/L，而肌肉浓度分别为21±9、20±4和25±7 nmol/g，肌肉中的总维生素B-6分别为28±10、27±4和35±10 nmol/g。这些数据进一步证实，骨骼肌中的维生素B-6储备对消耗具有抗性。它们还表明，在体重恒定的人类中，补充维生素B-6与肌肉中维生素B-6的显著增加无关。
