School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Bioscience, Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
ACS Appl Mater Interfaces. 2009 Dec;1(12):2747-54. doi: 10.1021/am9005435.
Thin films assembled from microgel building blocks have been constructed using a simple, high-throughput, and reproducible centrifugation (or "active") deposition technique. When compared to a common passive adsorption method (e.g., dip coating), microgels that are actively deposited onto a surface have smaller footprints and are more closely packed. Under both active and passive deposition conditions, the microgel footprint areas decrease during deposition. However, under active deposition, the microgel footprint appears to decrease continually and to a greater degree over the course of the deposition, forming a tightly packed, homogeneous film. Taking advantage of the rapid and uniform assembly of these films, we demonstrate the use of active deposition toward the fabrication of polyelectrolyte multilayers containing anionic microgels and a cationic linear polymer. Microgel multilayers successfully demonstrated effective blocking of the underlying substrate toward macrophage adhesion, which is a highly sought-after property for modulating the inflammatory response to an implanted biomaterial.
使用简单、高通量且可重复的离心（或“主动”）沉积技术，从微凝胶构建块组装了薄膜。与常见的被动吸附方法（例如浸涂）相比，主动沉积到表面上的微凝胶具有更小的足迹和更紧密的堆积。在主动和被动沉积条件下，沉积过程中微凝胶的足迹面积都会减小。但是，在主动沉积下，微凝胶的足迹似乎会持续不断地减小，并且在沉积过程中减小的程度更大，从而形成紧密堆积的均匀薄膜。利用这些薄膜的快速和均匀组装，我们展示了主动沉积在制备含有阴离子微凝胶和阳离子线性聚合物的聚电解质多层膜中的应用。微凝胶多层膜成功地证明了对底层基质的有效阻挡作用，以阻止巨噬细胞黏附，这是一种非常理想的特性，可以调节对植入生物材料的炎症反应。
