通过自动顶空毛细管气相色谱法诊断菌血症
Diagnosis of bacteraemia by automated head-space capillary gas chromatography.
作者信息
Larsson L, Mårdh P A, Odham G, Carlsson M L
出版信息
J Clin Pathol. 1982 Jul;35(7):715-8. doi: 10.1136/jcp.35.7.715.
Abstract
Blood cultures from 196 patients with suspected bacteraemia or septicaemia were analysed by automated head-space gas chromatography, using a 25 m fused silica capillary column, when turbidity indicated growth. Gas chromatography correctly identified 105 cultures as positive and 71 correctly as negative. No false-positive results were obtained. Of the 20 false-negative chromatographic results, Staphylococcus spp accounted for 14. Automated head-space gas chromatography is quicker, easier and more efficient than other gas chromatographic techniques for the evaluation of blood cultures.
摘要
对196例疑似菌血症或败血症患者的血培养样本进行分析，当出现浑浊提示有细菌生长时，使用一根25米的熔融石英毛细管柱，通过自动顶空气相色谱法进行检测。气相色谱法正确鉴定出105份培养物为阳性，71份为阴性，未出现假阳性结果。在20份色谱检测假阴性结果中，葡萄球菌属占14份。对于血培养评估，自动顶空气相色谱法比其他气相色谱技术更快、更简便且更高效。
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