通过气液色谱法快速初步鉴定血培养中的厌氧菌。
Rapid presumptive identification of anaerobes in blood cultures by gas-liquid chromatography.
作者信息
Sondag J E, Ali M, Murray P R
出版信息
J Clin Microbiol. 1980 Mar;11(3):274-7. doi: 10.1128/jcm.11.3.274-277.1980.
Abstract
Production of volatile and nonvolatile metabolic acids in blood culture broths by aerobic, facultative anaerobic, and obligate anaerobic bacteria was analyzed by gas-liquid chromatography. Anaerobic blood culture isolates were presumptively identified by the qualitative analysis of volatile fatty acids. Isolates, with a characteristic Gram stain reaction and cellular morphology, were identified by the following acid patterns: Bacteriodes fragilis group with acetic and propionic acids; Fusobacterium with acetic, butyric, and usually propionic acids; Veillonella with acetic and propionic acids; gram-positive cocci with acetic and butyric acids; and Clostridium with acetic and butyric acids.
摘要
采用气液色谱法分析了需氧菌、兼性厌氧菌和专性厌氧菌在血培养肉汤中挥发性和非挥发性代谢酸的产生情况。通过对挥发性脂肪酸的定性分析对厌氧血培养分离株进行初步鉴定。根据以下酸模式对具有特征性革兰氏染色反应和细胞形态的分离株进行鉴定：脆弱拟杆菌群产生乙酸和丙酸；梭杆菌产生乙酸、丁酸，通常还有丙酸；韦荣球菌产生乙酸和丙酸；革兰氏阳性球菌产生乙酸和丁酸；梭菌产生乙酸和丁酸。
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