Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
Nat Methods. 2010 May;7(5):383-5. doi: 10.1038/nmeth.1446. Epub 2010 Apr 4.
We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.
我们描述了一种通过将五种稳定同位素标记的氨基酸在细胞培养中的细胞系混合物（SILAC）与人类癌组织相结合来准确定量人肿瘤蛋白质组的方法。 这产生了数十万种同位素标记的肽，以适量作为基于质谱分析的内标。 通过将标记与测量分离，这种超级 SILAC 方法拓宽了 SILAC 蛋白质组学的范围。
