Department of Otorhinolaryngology, Medical Faculty, Firat University, 23119, Elazig, Turkey.
Eur Arch Otorhinolaryngol. 2010 Sep;267(9):1429-35. doi: 10.1007/s00405-010-1242-y. Epub 2010 Apr 3.
In this study, we assessed the effects of halofuginone and methylprednisolone on hypopharyngeal and esophageal stricture that can develop following radiation to the head and neck of rats. Rats were divided into four groups randomly and 18 Gy radiation was given to the head and neck regions of all rats except the control group. Group 1 (Control Group): No radiation or drugs were administered. Group 2 (Radiation Group): only radiation was applied without any drugs. Group 3 (Halofuginone Group): halofuginone 100 microg/kg per day was given intraperitoneally. Group 4 (Methylprednisolone Group): methylprednisolone 1 mg/kg per day was administered intramuscularly. In all groups, 90 days after application of radiation, sections of the proximal esophagus and hypopharynx were examined for fibrosis, fibroblast proliferation, vascularization, epithelial atypia, necrosis, polymorphonuclear leukocytes, mononuclear cells, and stenosis index by light microscope and the hydroxyproline levels were assessed biochemically. Fibrosis, epithelial atypia and hydroxyproline levels were found to be significantly higher in the radiation group compared to the control group (P < 0.05). We did not observe fibrosis in either the halofuginone or the control groups. Fibrosis was also significantly lower in the methylprednisolone group than the radiation group (P < 0.05). The differences of the stenosis index scores between the groups were not statistically significant (P < 0.05). Vascularization was similar in all groups. We think that especially halofuginone is a drug that can be used safely to prevent fibrosis due to radiotherapy, but further studies are needed.
在这项研究中，我们评估了卤夫酮和甲基强的松龙对大鼠头颈部放疗后可能发生的下咽和食管狭窄的影响。大鼠随机分为四组，除对照组外，所有大鼠的头颈部均给予 18Gy 照射。第 1 组（对照组）：未给予放射治疗或药物治疗。第 2 组（放射组）：仅给予放射治疗，无任何药物。第 3 组（卤夫酮组）：每天腹腔内给予卤夫酮 100μg/kg。第 4 组（甲基强的松龙组）：每天肌肉注射甲基强的松龙 1mg/kg。所有组大鼠在照射后 90 天，用光镜观察近端食管和下咽的纤维化、成纤维细胞增殖、血管生成、上皮异型性、坏死、多形核白细胞、单核细胞和狭窄指数，并通过生物化学方法评估羟脯氨酸水平。与对照组相比，放射组的纤维化、上皮异型性和羟脯氨酸水平明显升高（P<0.05）。我们在卤夫酮组或对照组中均未观察到纤维化。与放射组相比，甲基强的松龙组的纤维化也明显降低（P<0.05）。各组狭窄指数评分的差异无统计学意义（P<0.05）。各组血管生成相似。我们认为，特别是卤夫酮是一种可安全用于预防放疗引起的纤维化的药物，但需要进一步研究。
