Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0448, USA.
Phys Rev Lett. 2010 Mar 12;104(10):108301. doi: 10.1103/PhysRevLett.104.108301.
Single-molecule force spectroscopy provides a powerful approach for investigating molecular transitions along specific reaction coordinates. Here, we present a general analytical model for extracting the intrinsic rates and activation free energies from force measurements on single molecules that is applicable to a broad range of pulling speeds and device stiffnesses. This model relaxes existing limitations to perform force measurements with soft pulling devices for proper theoretical analyses and, in fact, allows experiments to specifically exploit device stiffness as a control parameter in addition to pulling speed for a reliable estimation of energetic and kinetic parameters.
单分子力谱学为研究沿着特定反应坐标的分子转变提供了一种强大的方法。在这里，我们提出了一种从单分子力测量中提取固有速率和激活自由能的通用分析模型，该模型适用于广泛的拉伸速度和器件刚度范围。该模型放宽了现有限制，使软拉伸装置能够进行适当的理论分析，实际上，允许实验将器件刚度作为除拉伸速度之外的另一个控制参数来进行力测量，从而可靠地估计能量和动力学参数。
