Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey.
Nanotechnology. 2010 Apr 30;21(17):175104. doi: 10.1088/0957-4484/21/17/175104. Epub 2010 Apr 6.
Silver nanoparticles (AgNPs) are widely used in household products and in medicine due to their antibacterial and to wound healing properties. In recent years, there is also an effort for their use in biomedical imaging and photothermal therapy. The primary reason behind the effort for their utility in biomedicine and therapy is their unique plasmonic properties and easy surface chemistry for a variety of functionalizations. In this study, AgNPs modified with glucose, lactose, oligonucleotides and combinations of these ligands are investigated for their cytotoxicity and cellular uptake in living non-cancer (L929) and cancer (A549) cells. It is found that the chemical nature of the ligand strongly influences the toxicity and cellular uptake into the model cells. While the lactose-and glucose-modified AgNPs enter the L929 cells at about the same rate, a significant increase in the rate of lactose-modified AgNPs into the A549 cells is observed. The binding of oligonucleotides along with the carbohydrate on the AgNP surfaces influences the differential uptake rate pattern into the cells. The cytotoxicity study with the modified AgNPs reveals that only naked AgNPs influence the viability of the A549 cells. The findings of this study may provide the key to developing effective applications in medicine such as cancer therapy.
银纳米粒子（AgNPs）由于其抗菌和促进伤口愈合的特性，被广泛应用于家庭产品和医学领域。近年来，它们也被用于生物医学成像和光热治疗。将它们应用于生物医学和治疗的主要原因是它们具有独特的等离子体特性和易于进行各种功能化的表面化学性质。在这项研究中，研究了用葡萄糖、乳糖、寡核苷酸以及这些配体的组合修饰的 AgNPs 在活非癌细胞（L929）和癌细胞（A549）中的细胞毒性和细胞摄取。结果发现，配体的化学性质强烈影响毒性和模型细胞的摄取。虽然乳糖和葡萄糖修饰的 AgNPs 进入 L929 细胞的速度大致相同，但观察到乳糖修饰的 AgNPs 进入 A549 细胞的速度显著增加。寡核苷酸与 AgNP 表面上的碳水化合物结合，影响了进入细胞的不同摄取率模式。用修饰的 AgNPs 进行的细胞毒性研究表明，只有裸露的 AgNPs 会影响 A549 细胞的活力。这项研究的结果可能为开发癌症治疗等医学领域的有效应用提供关键。
