Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan.
Meat Sci. 2010 Jun;85(2):285-8. doi: 10.1016/j.meatsci.2010.01.015. Epub 2010 Jan 18.
In Japan, Japanese Black and Holstein cattle are appreciated as popular sources of meat, and imported beef from Australia and the United States is also in demand in the meat industry. Since the BSE outbreak, the problem of false sales has arisen: imported beef has sometimes been mislabeled as domestic beef due to consumer concerns. A method is needed to correctly discriminate between Japanese and imported cattle for food safety. The objective of this study was to develop breed discrimination markers between Japanese and US cattle using a 50K SNP array. As a result, five US-specific markers (BISNP7, BISNP15, BISNP21, BISNP23, and BISNP26) were developed with allelic frequencies that ranged from 0.102 (BISNP15) to 0.250 (BISNP7) and averaged 0.184. The combined use of the five markers would permit discrimination between Japanese and US cattle with a probability of identification of 0.858. This result indicates the potential of the bovine 50K SNP array as a powerful tool for developing breed identification markers. These markers would contribute to the prevention of falsified beef displays in Japan.
在日本，日本黑毛和牛和荷斯坦牛被视为受欢迎的肉类来源，来自澳大利亚和美国的进口牛肉在肉类行业也有需求。自疯牛病爆发以来，出现了虚假销售的问题：由于消费者的担忧，进口牛肉有时会被错误地贴上国产牛肉的标签。因此，需要一种方法来正确区分食品安全性方面的日本牛和进口牛。本研究的目的是使用 50K SNP 阵列开发日本和美国牛之间的品种鉴别标记。结果，开发了五个美国特有的标记物（BISNP7、BISNP15、BISNP21、BISNP23 和 BISNP26），等位基因频率范围为 0.102（BISNP15）至 0.250（BISNP7），平均为 0.184。这五个标记物的联合使用可以将日本和美国牛区分开来，识别概率为 0.858。这一结果表明，牛 50K SNP 阵列作为开发品种识别标记物的有力工具具有潜力。这些标记物将有助于防止日本虚假牛肉展示。
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