Kawaguchi Fuki, Kitamura Yuto, Nakajima Ryuji, Takahashi Masayoshi, Goto Hiroaki, Washida Yuichi, Yamamoto Yoshikazu, Sasazaki Shinji, Mannen Hideyuki
Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
DNA Chip Business Promotion Division, Toshiba Corporation, Tokyo, Japan.
Anim Sci J. 2018 Jan;89(1):257-258. doi: 10.1111/asj.12938. Epub 2017 Oct 30.
The objective of this study was to discriminate between original Japanese and Australian Wagyu beef, which is sold in the Singapore markets, using six previously developed DNA markers. To effectively evaluate the six markers for breed identification, the probability of identification as Australian Wagyu beef was calculated based on the estimated allele frequencies using 130 Australian Wagyu individuals. The combined use of six markers would allow the discrimination of Australian Wagyu beef with an estimated probability of 0.776. The probability to discriminate Australian Wagyu from Japanese Wagyu beef was sufficiently high. In addition, Australian Wagyu has maternal mitochondrial DNA of Bos indicus cattle with moderate high frequency of 0.377. The DNA marker system could also be used as a deterrent force against false sales, and contribute to the reduction and prevention of incorrect or falsified labeling of beef.
本研究的目的是利用六个先前开发的DNA标记，区分在新加坡市场上销售的原产于日本和澳大利亚的和牛。为了有效评估这六个用于品种鉴定的标记，基于130头澳大利亚和牛个体的估计等位基因频率，计算了鉴定为澳大利亚和牛的概率。六个标记的联合使用将能够以0.776的估计概率区分澳大利亚和牛。将澳大利亚和牛与日本和牛区分开的概率足够高。此外，澳大利亚和牛具有中等高频的印度瘤牛母系线粒体DNA，频率为0.377。该DNA标记系统还可作为打击虚假销售的威慑力量，并有助于减少和防止牛肉标签错误或造假。
