Sasazaki S, Mutoh H, Tsurifune K, Mannen H
Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe-shi, Kobe 657-8501, Japan.
Meat Sci. 2007 Oct;77(2):161-6. doi: 10.1016/j.meatsci.2007.02.024. Epub 2007 Mar 12.
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese and Australian beef. Two Bos indicus-specific markers and MC1R marker were used as possible candidate markers. Amplified fragment length polymorphism method was employed to develop additional candidate markers. The 1564 primer combinations provided three markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. In these markers, the allele frequencies in cattle from both countries were investigated for discrimination ability using PCR-RFLP. The probability of identifying Australian beef was 0.933 and probability of misjudgment was 0.017 using six selected markers. These markers could be useful for discriminating between Japanese and Australian beef and would contribute to the prevention of falsified breed labeling of meat.
在肉类行业，食品标签中需要正确的品种信息以确保肉质。基因标记为识别品种提供确证。本文描述了用于区分日本牛肉和澳大利亚牛肉的DNA标记的开发。使用两个印度瘤牛特异性标记和MC1R标记作为可能的候选标记。采用扩增片段长度多态性方法开发其他候选标记。1564个引物组合产生了三个标记，这些标记被转化为单核苷酸多态性标记用于高通量基因分型。在这些标记中，使用PCR-RFLP研究了两国牛群中的等位基因频率以评估其鉴别能力。使用六个选定的标记，识别澳大利亚牛肉的概率为0.933，误判概率为0.017。这些标记可用于区分日本牛肉和澳大利亚牛肉，并将有助于防止肉类品种标签造假。
