用于检测活细胞内铜（I）的高灵敏度荧光探针的研制。
Development of highly sensitive fluorescent probes for detection of intracellular copper(I) in living systems.
机构信息
Graduate School of Human and Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
出版信息
J Am Chem Soc. 2010 May 5;132(17):5938-9. doi: 10.1021/ja100714p.
We describe here the new copper-selective fluorescent probes FluTPA1 and FluTPA2, in which the tetradentate ligand tris[(2-pyridyl)methyl]amine (TPA) is connected to a reduced form of a fluorescein platform through a benzyl ether linkage. These probes selectively react with copper ions in the presence of submillimolar glutathione and emit intense green fluorescence from the reaction product, O-methylfluorescein. Confocal images of live cells further show that FluTPA2 is membrane-permeable and allows visualization of Cu(I) present in living cells.
我们在这里描述了新的铜选择性荧光探针 FluTPA1 和 FluTPA2，其中四齿配体三[(2-吡啶基)甲基]胺(TPA)通过苄基醚键连接到荧光素平台的还原形式上。这些探针在亚毫摩尔谷胱甘肽存在下选择性地与铜离子反应，并从反应产物 O-甲基荧光素发出强烈的绿色荧光。活细胞的共焦图像进一步表明，FluTPA2 具有膜透过性，可用于可视化活细胞中存在的 Cu(I)。
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