Institute of Microbiology, University Hospital Center and University of Lausanne, Bugnon 48, 1011 Lausanne, Switzerland.
Methods. 2011 Jan;53(1):39-46. doi: 10.1016/j.ymeth.2010.04.004. Epub 2010 Apr 10.
The integration of the Human Immunodeficiency Virus (HIV) genetic information into the host genome is fundamental for its replication and long-term persistence in the host. Isolating and characterizing the integration sites can be useful for obtaining data such as identifying the specific genomic location of integration or understanding the forces dictating HIV integration site selection. The methods outlined in this article describe a highly efficient and precise technique for identifying HIV integration sites in the host genome on a small scale using molecular cloning techniques and standard sequencing or on a massive scale using 454 pyrosequencing.
人类免疫缺陷病毒（HIV）的遗传信息整合到宿主基因组中是其复制和长期在宿主中持续存在的基础。分离和鉴定整合位点对于获得数据非常有用，例如确定整合的特定基因组位置或了解决定 HIV 整合位点选择的力量。本文中概述的方法描述了一种使用分子克隆技术和标准测序在小规模上，或使用 454 焦磷酸测序在大规模上鉴定宿主基因组中 HIV 整合位点的高效、精确技术。
