Suppr超能文献

金属依赖性抑制甘油醛-3-磷酸脱氢酶 II:一种调节酶活性的可能机制。

Metal-dependent inhibition of glyoxalase II: a possible mechanism to regulate the enzyme activity.

机构信息

IBR (Instituto de Biología Molecular y Celular de Rosario), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK, Rosario, Argentina.

出版信息

J Inorg Biochem. 2010 Jul;104(7):726-31. doi: 10.1016/j.jinorgbio.2010.03.005. Epub 2010 Mar 20.

Abstract

Glyoxalase II (GLX2, EC 3.1.2.6., hydroxyacylglutathione hydrolase) is a metalloenzyme involved in crucial detoxification pathways. Different studies have failed in identifying the native metal ion of this enzyme, which is expressed with iron, zinc and/or manganese. Here we report that GloB, the GLX2 from Salmonella typhimurium, is differentially inhibited by glutathione (a reaction product) depending on the bound metal ion, and we provide a structural model for this inhibition mode. This metal-dependent inhibition was shown to occur in metal-enriched forms of the enzyme, complementing the spectroscopic data. Based on the high levels of free glutathione in the cell, we suggest that the expression of the different metal forms of GLX2 during Salmonella infection could be exploited as a mechanism to regulate the enzyme activity.

摘要

醛糖二酸酶 II(GLX2,EC 3.1.2.6.,羟酰基谷胱甘肽水解酶)是一种参与关键解毒途径的金属酶。不同的研究未能确定该酶的天然金属离子,该酶表达为铁、锌和/或锰。在这里,我们报告称,来自伤寒沙门氏菌的 GLX2 的 GloB 会根据结合的金属离子而被谷胱甘肽(反应产物)不同程度地抑制,并且我们提供了这种抑制模式的结构模型。这种金属依赖性抑制发生在酶的金属富集形式中,补充了光谱数据。基于细胞中高水平的游离谷胱甘肽,我们建议在沙门氏菌感染期间表达 GLX2 的不同金属形式可以作为一种调节酶活性的机制。

相似文献

1
Metal-dependent inhibition of glyoxalase II: a possible mechanism to regulate the enzyme activity.
J Inorg Biochem. 2010 Jul;104(7):726-31. doi: 10.1016/j.jinorgbio.2010.03.005. Epub 2010 Mar 20.
4
Structural studies on a mitochondrial glyoxalase II.
J Biol Chem. 2005 Dec 9;280(49):40668-75. doi: 10.1074/jbc.M509748200. Epub 2005 Oct 14.
8
Converting GLX2-1 into an active glyoxalase II.
Biochemistry. 2010 Sep 21;49(37):8228-36. doi: 10.1021/bi1010865.
9
Explaining the inhibition of glyoxalase II by 9-fluorenylmethoxycarbonyl-protected glutathione derivatives.
Arch Biochem Biophys. 2003 Jun 15;414(2):271-8. doi: 10.1016/s0003-9861(03)00193-0.
10
Human glyoxalase II contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme.
Biochemistry. 2009 Jun 16;48(23):5426-34. doi: 10.1021/bi9001375.

引用本文的文献

1
Deciphering novel enzymatic and non-enzymatic lysine lactylation in .
Emerg Microbes Infect. 2025 Dec;14(1):2475838. doi: 10.1080/22221751.2025.2475838. Epub 2025 Mar 19.
2
Glyoxalase 2: Towards a Broader View of the Second Player of the Glyoxalase System.
Antioxidants (Basel). 2022 Oct 28;11(11):2131. doi: 10.3390/antiox11112131.
3
A Nitrogen Metabolic Enzyme Provides Fitness Advantage by Promoting Utilization of Microbiota-Derived Carbon Source.
ACS Infect Dis. 2021 May 14;7(5):1208-1220. doi: 10.1021/acsinfecdis.0c00836. Epub 2021 Apr 14.
5
Converting GLX2-1 into an active glyoxalase II.
Biochemistry. 2010 Sep 21;49(37):8228-36. doi: 10.1021/bi1010865.

本文引用的文献

1
Human glyoxalase II contains an Fe(II)Zn(II) center but is active as a mononuclear Zn(II) enzyme.
Biochemistry. 2009 Jun 16;48(23):5426-34. doi: 10.1021/bi9001375.
2
Reaction mechanism of the binuclear zinc enzyme glyoxalase II - A theoretical study.
J Inorg Biochem. 2009 Feb;103(2):274-81. doi: 10.1016/j.jinorgbio.2008.10.016. Epub 2008 Oct 31.
3
Protein and nucleotide damage by glyoxal and methylglyoxal in physiological systems--role in ageing and disease.
Drug Metabol Drug Interact. 2008;23(1-2):125-50. doi: 10.1515/dmdi.2008.23.1-2.125.
4
Microbial glyoxalase enzymes: metalloenzymes controlling cellular levels of methylglyoxal.
Drug Metabol Drug Interact. 2008;23(1-2):29-50. doi: 10.1515/dmdi.2008.23.1-2.29.
5
Molecular enzymology of the glyoxalase system.
Drug Metabol Drug Interact. 2008;23(1-2):13-27. doi: 10.1515/dmdi.2008.23.1-2.13.
7
Structure and specificity of a quorum-quenching lactonase (AiiB) from Agrobacterium tumefaciens.
Biochemistry. 2007 Oct 23;46(42):11789-99. doi: 10.1021/bi7012849. Epub 2007 Sep 28.
9
Metallo-beta-lactamases (classification, activity, genetic organization, structure, zinc coordination) and their superfamily.
Biochem Pharmacol. 2007 Dec 15;74(12):1686-701. doi: 10.1016/j.bcp.2007.05.021. Epub 2007 Jun 2.
10
Escherichia coli glyoxalase II is a binuclear zinc-dependent metalloenzyme.
Arch Biochem Biophys. 2007 Mar 1;459(1):20-6. doi: 10.1016/j.abb.2006.11.024. Epub 2006 Dec 6.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验