Department of Pathology, School of Medicine, University of São Paulo, São Paulo, Brazil.
Toxicol Sci. 2010 Jul;116(1):67-78. doi: 10.1093/toxsci/kfq107. Epub 2010 Apr 12.
Analysis of fuel emissions is crucial for understanding the pathogenesis of mortality because of air pollution. The objective of this study is to assess cardiovascular and inflammatory toxicity of diesel and biodiesel particles. Mice were exposed to fuels for 1 h. Heart rate (HR), heart rate variability, and blood pressure were obtained before exposure, as well as 30 and 60 min after exposure. After 24 h, bronchoalveolar lavage, blood, and bone marrow were collected to evaluate inflammation. B100 decreased the following emission parameters: mass, black carbon, metals, CO, polycyclic aromatic hydrocarbons, and volatile organic compounds compared with B50 and diesel; root mean square of successive differences in the heart beat interval increased with diesel (p < 0.05) compared with control; low frequency increased with diesel (p < 0.01) and B100 (p < 0.05) compared with control; HR increased with B100 (p < 0.05) compared with control; mean corpuscular volume increased with B100 compared with diesel (p < 0.01), B50, and control (p < 0.001); mean corpuscular hemoglobin concentration decreased with B100 compared with B50 (p < 0.001) and control (p < 0.05); leucocytes increased with B50 compared with diesel (p < 0.05); platelets increased with B100 compared with diesel and control (p < 0.05); reticulocytes increased with B50 compared with diesel, control (p < 0.01), and B100 (p < 0.05); metamyelocytes increased with B50 and B100 compared with diesel (p < 0.05); neutrophils increased with diesel and B50 compared with control (p < 0.05); and macrophages increased with diesel (p < 0.01), B50, and B100 (p < 0.05) compared with control. Biodiesel was more toxic than diesel because it promoted cardiovascular alterations as well as pulmonary and systemic inflammation.
分析燃料排放对于了解空气污染导致的死亡率发病机制至关重要。本研究旨在评估柴油和生物柴油颗粒的心血管和炎症毒性。小鼠暴露于燃料 1 小时。在暴露前以及暴露后 30 分钟和 60 分钟时，获取心率（HR）、心率变异性和血压。24 小时后，收集支气管肺泡灌洗液、血液和骨髓以评估炎症。与 B50 和柴油相比，B100 降低了以下排放参数：质量、黑碳、金属、CO、多环芳烃和挥发性有机化合物；与对照相比，柴油（p < 0.05）导致窦性心动间隔的均方根差增加；与对照相比，柴油（p < 0.01）和 B100（p < 0.05）低频增加；与对照相比，B100 导致 HR 增加（p < 0.05）；与柴油相比，B100 导致平均红细胞体积增加（p < 0.01），B50 和对照（p < 0.001）；与 B50 相比，B100 导致平均红细胞血红蛋白浓度降低（p < 0.001）和对照（p < 0.05）；与柴油相比，B50 导致白细胞增多（p < 0.05）；与柴油和对照相比，B100 导致血小板增加（p < 0.05）；与柴油、对照（p < 0.01）和 B100（p < 0.05）相比，B50 导致网织红细胞增加；与柴油相比，B50 和 B100 导致早幼粒细胞增加（p < 0.05）；与对照相比，柴油和 B50 导致中性粒细胞增加（p < 0.05）；与对照相比，柴油（p < 0.01）、B50 和 B100 导致巨噬细胞增加（p < 0.05）。生物柴油比柴油毒性更大，因为它促进了心血管改变以及肺和全身炎症。
