Department of Biochemistry, Meram Medical Faculty, Selcuk University, Konya, Turkey.
J Med Food. 2010 Jun;13(3):725-8. doi: 10.1089/jmf.2009.0232.
Procyanidins, a group of flavonoids, are oligomeric forms of catechins that are abundant in red wine, grapes, cocoa, and apples. Paraoxonase acts as an antioxidant enzyme and protects low-density lipoprotein-cholesterol against oxidation. In our study we aimed to evaluate the effects of grape seed extract (GSE) on paraoxonase activities in streptozotocin-induced diabetic rats. Our study included four groups of rats: Group I (n = 8), control; Group II (n = 10), GSE-supplemented; Group III (n = 6), streptozotocin-induced diabetic; and Group IV (n = 7), GSE-supplemented diabetic rats. Serum paraoxonase activities were determined with a spectrophotometric method. Paraoxonase activities in Group III were significantly lower than in the other three groups (P < .001, P < .001, and P = .005 for Groups I, II, and IV, respectively), and Group IV showed increased paraoxonase activities compared to Group III (P = .005). This is the first study to show an association between paraoxonase status and GSE supplementation and demonstrated that GSE increased paraoxonase activities. This beneficial effect of GSE was more obvious in the diabetic group, which was more prone to atherosclerotic events compared to the healthy population.
原花青素是黄酮类化合物的一组，是在红酒、葡萄、可可和苹果中丰富的儿茶素的低聚物形式。对氧磷酶作为抗氧化酶，可保护低密度脂蛋白胆固醇免受氧化。在我们的研究中，我们旨在评估葡萄籽提取物（GSE）对链脲佐菌素诱导的糖尿病大鼠对氧磷酶活性的影响。我们的研究包括四组大鼠：第 I 组（n = 8），对照组；第 II 组（n = 10），补充 GSE；第 III 组（n = 6），链脲佐菌素诱导的糖尿病；第 IV 组（n = 7），补充 GSE 的糖尿病大鼠。使用分光光度法测定血清对氧磷酶活性。第 III 组的对氧磷酶活性明显低于其他三组（P <.001，P <.001 和 P =.005 分别为第 I、II 和 IV 组），并且第 IV 组的对氧磷酶活性比第 III 组升高（P =.005）。这是第一项表明对氧磷酶状态与 GSE 补充之间存在关联的研究，并表明 GSE 增加了对氧磷酶活性。与健康人群相比，GSE 的这种有益作用在糖尿病组中更为明显，因为糖尿病组更容易发生动脉粥样硬化事件。
