Yanagisawa M, Inoue A, Ishikawa T, Kasuya Y, Kimura S, Kumagaye S, Nakajima K, Watanabe T X, Sakakibara S, Goto K
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6964-7. doi: 10.1073/pnas.85.18.6964.
Endothelin is a potent vasoconstrictor/pressor peptide, which we recently characterized from the conditioned culture medium of porcine aortic endothelial cells. We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelin; 15 residues of rat endothelin were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. Synthetic rat endothelin was then prepared according to its deduced amino acid sequence. This synthetic peptide had (i) potent vasoconstrictor activity in the rat aortic strip and in perfused rat heart and (ii) a characteristically long-lasting in vivo pressor activity by intraaortic bolus injection in the conscious rat.
内皮素是一种强效血管收缩剂/升压肽，我们最近从猪主动脉内皮细胞的条件培养基中对其进行了鉴定。我们在此报告大鼠内皮素基因的克隆及部分测序。核苷酸序列预测出一种21个氨基酸残基的肽，与猪内皮素相似但又不同；大鼠内皮素的15个残基相同，3个残基被化学性质与猪肽中氨基酸残基相似的氨基酸残基所取代。然后根据推导的氨基酸序列制备了合成大鼠内皮素。这种合成肽（i）在大鼠主动脉条和灌注大鼠心脏中具有强效血管收缩活性，（ii）通过在清醒大鼠体内进行主动脉推注注射具有特征性的持久升压活性。
