Bobrow L G, Happerfield L, Patel K
Department of Histopathology, ICRF Human Tumour Immunology Group, London, UK.
Br J Cancer Suppl. 1991 Jun;14:56-8.
The presence and distribution of antigens recognised by the antibodies submitted to the Second International Workshop on Small Cell Lung Cancer Antigens has been mapped on human foetal lung and kidney using a streptavidin immunoperoxidase technique on frozen tissue sections. Thirteen of the antibodies showed no staining at all on these tissues and five showed staining of all structures in all tissues of equal intensity. The staining patterns of the remaining antibodies could be divided into several groups. All the cluster 1 antibodies recognising the neural cell adhesion molecule (NCAM) gave a distinctive staining pattern in both tissues. The cluster 2 antibodies and several groups of unclustered antibodies gave varying pure epithelial patterns of staining. The cluster w4 and 5 antibodies gave an interesting staining pattern which included some of the distinctive features of the cluster 1 pattern plus some epithelial expression. Various less distinctive patterns were also observed. Serial sections of foetal lung which had been stained with NCC-LU-246 (cluster 1), anti-desmin, and anti-neurofilament antibodies were also studied to attempt to determine the nature of the cells expressing NCAM in the foetal lung.
在第二届小细胞肺癌抗原国际研讨会上提交的抗体所识别的抗原的存在情况及分布，已通过链霉亲和素免疫过氧化物酶技术在冷冻组织切片上，绘制于人类胎儿肺和肾中。其中13种抗体在这些组织上完全没有染色，5种抗体对所有组织中的所有结构均呈现强度相等的染色。其余抗体的染色模式可分为几组。所有识别神经细胞黏附分子（NCAM）的第1组抗体在两种组织中均呈现独特的染色模式。第2组抗体和几组未分组的抗体呈现出不同的单纯上皮染色模式。第w4和5组抗体呈现出一种有趣的染色模式，其中包括第1组模式的一些独特特征以及一些上皮表达。还观察到了各种不太明显的模式。还研究了用NCC-LU-246（第1组）、抗结蛋白和抗神经丝抗体染色的胎儿肺连续切片，以试图确定胎儿肺中表达NCAM的细胞的性质。
