肉桂醛、其配体和 Ni(II)配合物的抗真菌活性：增加环和侧链的影响。

Anticandidal activity of cinnamaldehyde, its ligand and Ni(II) complex: effect of increase in ring and side chain.

机构信息

Department of Biosciences, Jamia Millia Islamia (Central University), New Delhi, India.

出版信息

Microb Pathog. 2010 Sep;49(3):75-82. doi: 10.1016/j.micpath.2010.03.013. Epub 2010 Apr 23.

DOI:10.1016/j.micpath.2010.03.013

PMID:20399846

Abstract

To increase efficacy of cinnamaldehyde as an antimycotic agent, N, N'- Bis (trans-cinnamadehyde) ethylenediimine [C(20)H(20)N(2)] and Ni(II) complex of the type [Ni(C(40)H(40)N(4))Cl(2)] have been synthesized. The ligand [P] and Ni(II) complex have been characterized on the basis of elemental analysis, FTIR, ESI- MS, IR, (1)H NMR, UV-Vis spectroscopic techniques, conductivity and magnetic measurements. MIC of cinnamaldehyde against clinical isolate of Candida albicans and Candida tropicalis was 400 microg/ml and 500 microg/ml, respectively. Synthesized ligand has markedly reduced MIC; 200 microg/ml and 300 microg/ml whereas Ni(II) complex of ligand displayed MIC of 90 microg/ml and 120 microg/ml. Growth and sensitivity of the organisms were effected by ligand & complex at significantly reduced concentration. Plasma membrane ATPase activity and ergosterol content have been investigated as site of action. Result obtained indicates ergosterol biosynthesis pathway as site of action of cinnamaldehyde, synthesized ligand and its Ni(II) complex.

摘要

为了提高肉桂醛作为抗真菌剂的疗效，合成了 N, N'-双（反式肉桂醛）乙二亚胺 [C(20)H(20)N(2)] 和 Ni(II)配合物[Ni(C(40)H(40)N(4))Cl(2)]。根据元素分析、FTIR、ESI-MS、IR、(1)H NMR、UV-Vis 光谱技术、电导率和磁测量对配体[P]和 Ni(II)配合物进行了表征。肉桂醛对临床分离的白色念珠菌和热带假丝酵母的 MIC 分别为 400 μg/ml 和 500 μg/ml。合成的配体显著降低了 MIC；200 μg/ml 和 300 μg/ml，而配体的 Ni(II)配合物则显示 MIC 为 90 μg/ml 和 120 μg/ml。配体和配合物在显著降低的浓度下影响了生物体的生长和敏感性。研究了质膜 ATP 酶活性和麦角固醇含量作为作用部位。结果表明，肉桂醛、合成配体及其 Ni(II)配合物的作用部位是麦角固醇生物合成途径。