School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, PR China.
J Pharm Biomed Anal. 2010 Nov 2;53(3):559-66. doi: 10.1016/j.jpba.2010.03.034. Epub 2010 Mar 31.
This paper was designed to study metabonomic characters of the nephrotoxicity induced by Morning Glory Seed (MGS), a well-known traditional Chinese medicine which was used for the treatment of edema, simple obesity and lung fever. Urinary samples from control and MGS treated rats were analyzed by ultra-performance liquid chromatography/mass spectrometry (UPLC-MS) in positive ionization mode. Blood biochemistry and histopathology were examined to identify specific changes of renal damage. The results affirmatively suggested that ethanol extract of Morning Glory Seed (EMGS), instead of water extract of Morning Glory Seed (WMGS), should be responsible for the nephrotoxicity caused by this herbal medicine. The UPLC-MS analysis revealed that the levels of 8 endogenous metabolites as biomarkers were significantly changed in urine from EMGS treated rats. The underlying regulations of EMGS-perturbed metabolic pathways were discussed according to the identified metabolites. The present study proves the potential of UPLC-MS based metabonomics in mapping metabolic response for toxicology.
本文旨在研究中药牵牛子（Morning Glory Seed，MGS）肾毒性的代谢组学特征。牵牛子是一种传统中药，常用于治疗水肿、单纯性肥胖和肺热等疾病。采用超高效液相色谱/质谱联用技术（UPLC-MS）在正离子模式下对对照组和 MGS 处理组大鼠的尿液样本进行分析。同时，检测血液生化学和组织病理学变化，以确定肾脏损伤的特异性变化。研究结果肯定地表明，牵牛子的乙醇提取物（EMGS）而不是水提取物（WMGS）应负责这种草药引起的肾毒性。UPLC-MS 分析显示，EMGS 处理大鼠尿液中 8 种内源性代谢物标志物的水平显著改变。根据鉴定出的代谢物，讨论了 EMGS 干扰代谢途径的潜在规律。本研究证明了基于 UPLC-MS 的代谢组学在毒理学中绘制代谢反应图谱的潜力。
