来自草生欧文氏菌的类胡萝卜素生成基因簇的定位及六个基因的功能鉴定。
Mapping of a carotenogenic gene cluster from Erwinia herbicola and functional identification of six genes.
作者信息
Schnurr G, Schmidt A, Sandmann G
机构信息
Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, F.R.G.
出版信息
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):157-61. doi: 10.1016/0378-1097(91)90151-y.
Six genes have been mapped and identified by hybridization or by carotenoid analysis of deletion mutants on a 9 kb chromosomal fragment originating from Erwinia herbicola Eho 10. These genes include crtB, E and I which have been formerly described for Rhodobacter, and three new ones: crtZ encoding lycopene cyclase, crtH for beta-carotene hydroxylase, and crtG for zeaxanthin glycosilase. Their arrangement on the plasmid has been elucidated.
通过对源自草生欧文氏菌Eho 10的9 kb染色体片段上的缺失突变体进行杂交或类胡萝卜素分析，已定位并鉴定出六个基因。这些基因包括先前在红细菌中描述过的crtB、E和I，以及三个新基因：编码番茄红素环化酶的crtZ、β-胡萝卜素羟化酶的crtH和玉米黄质糖基化酶的crtG。它们在质粒上的排列已得到阐明。
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