Van Dien Stephen J, Marx Christopher J, O'Brien Brooke N, Lidstrom Mary E
Department of Chemical Engineering. Department of Microbiology, University of Washington, Seattle, Washington 98195-2180, USA.
Appl Environ Microbiol. 2003 Dec;69(12):7563-6. doi: 10.1128/AEM.69.12.7563-7566.2003.
Genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. Four genes for putative phytoene desaturases were identified. A colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. Mutations in the other three did not affect color. The tetracycline marker was removed from the original transposon mutant, resulting in a pigment-free strain with wild-type growth properties useful as a tool for future experiments.
利用基因组搜索重建了兼性甲基营养型粉红色色素菌甲基营养型嗜甲基菌AM1中假定的类胡萝卜素生物合成途径。鉴定出了四个假定的八氢番茄红素去饱和酶基因。通过转座子诱变获得了一个无色突变体，插入位点显示在其中一个假定的八氢番茄红素去饱和酶基因中。另外三个基因的突变不影响颜色。从原始转座子突变体中去除了四环素标记，得到了一个无色素菌株，其具有野生型生长特性，可作为未来实验的工具。
