Mukaida H, Hirabayashi N, Hirai T, Iwata T, Saeki S, Toge T
Department of Surgery, Hiroshima University, Japan.
Int J Cancer. 1991 May 30;48(3):423-7. doi: 10.1002/ijc.2910480320.
The interactions between human cancer cells and primary cultured human fibroblasts without cell-to-cell contact were investigated using double soft-agar culture. Human fibroblasts obtained from different organs were cultured in monolayers and used after the 3rd or 4th passage. In double soft-agar culture, colony formations of cancer cells in the overlayer were stimulated or inhibited by the presence of various kinds of fibroblast in the underlayer. The growth of all cancer cells tested was always stimulated by the presence of fibroblasts obtained from an organ in which cancer cells had already developed, and inhibited by those from skin. However, fibroblast-conditioned media failed to affect cancer cell growth, either in MTT assay or in soft-agar culture. These results suggest that mutual growth reliance exists between human cancer cells and primary cultured fibroblasts by diffusible factors secreted by both cells (paracrine growth) and that mutual growth enhancement occurs between cancer cells and fibroblasts derived from tissues in which cancer cells had originated.
使用双层软琼脂培养法研究了人癌细胞与原代培养的人成纤维细胞之间无细胞间接触的相互作用。从不同器官获取的人成纤维细胞进行单层培养，并在传代3次或4次后使用。在双层软琼脂培养中，下层中各种成纤维细胞的存在会刺激或抑制上层癌细胞的集落形成。所测试的所有癌细胞的生长总是受到来自癌细胞已经发生的器官的成纤维细胞的刺激，并受到来自皮肤的成纤维细胞的抑制。然而，在MTT试验或软琼脂培养中，成纤维细胞条件培养基均未能影响癌细胞的生长。这些结果表明，人癌细胞与原代培养的成纤维细胞之间通过两种细胞分泌的可扩散因子（旁分泌生长）存在相互生长依赖，并且癌细胞与源自癌细胞起源组织的成纤维细胞之间会发生相互生长增强。
