Pandey Sarita, Tiwari Shalini, Bhattacharjee Poulami, Anand Abhishek, Pazhamala Lekha T, Yasin Jeshima K, Yadav Roshan Kumar
Department of Genetics and Plant Breeding, School of Agriculture and Allied Sciences, The Neotia University, West Bengal, India.
Department of Agricultural and Biosystem Engineering, South Dakota State University, Brookings, South Dakota, USA.
Plant Genome. 2025 Sep;18(3):e70027. doi: 10.1002/tpg2.70027.
Despite the rapid progress of proteomics in human and other model organisms, plant proteomics has advanced at a comparatively slower pace. This review aims to highlight the pioneering work on seed protein markers detected by employing gel electrophoresis primarily by a team of Indian scientists that paved the way for elucidation of intervarietal and interspecific variation, evolution, and phylogenetic relationship of species and their association with resistance to pest and diseases. Far from being replaced, gel electrophoresis remains as an excellent supporting and different approach, offering a pathway to a more profound visualization and understanding of the cell proteome. This review focuses on how, from a historical standpoint, gel electrophoresis has significantly contributed to plant proteomics and other biological research. Acknowledging the pioneering work on seed storage proteins, this review serves as both a congratulatory gesture and a tribute to the eminent scientist Prof. Chittaranjan Kole and his team who pioneered the strategy of seed protein electrophoresis in crop biology research. Their findings, both directly and indirectly, have proven invaluable, particularly for those who ventured into proteomics without easy reach to sophisticated and expensive instruments/equipment to pursue DNA-based genomics research. This gel electrophoresis-based plant proteomics review includes the evolution of gel-based proteomics, their contribution to crop biology research, and future directions. It stands not only as a retrospective analysis but also as a testament to the enduring significance of gel electrophoresis in shaping the landscape of crop proteomics.
尽管蛋白质组学在人类和其他模式生物中取得了快速进展，但植物蛋白质组学的发展速度相对较慢。本综述旨在突出印度科学家团队主要通过凝胶电泳检测种子蛋白质标记物的开创性工作，这些工作为阐明品种间和种间变异、进化以及物种的系统发育关系及其与病虫害抗性的关联铺平了道路。凝胶电泳远未被取代，仍然是一种出色的辅助和不同的方法，为更深入地可视化和理解细胞蛋白质组提供了途径。本综述重点关注从历史角度来看，凝胶电泳如何对植物蛋白质组学和其他生物学研究做出了重大贡献。在认可种子储存蛋白开创性工作的同时，本综述既是一种祝贺姿态，也是对杰出科学家奇特拉兰詹·科尔教授及其团队的致敬，他们在作物生物学研究中开创了种子蛋白质电泳策略。他们的发现，无论是直接还是间接，都已证明具有不可估量的价值，特别是对于那些在没有容易获得复杂且昂贵的仪器设备来进行基于DNA的基因组学研究的情况下涉足蛋白质组学的人。这篇基于凝胶电泳的植物蛋白质组学综述涵盖了基于凝胶的蛋白质组学的发展、它们对作物生物学研究的贡献以及未来方向。它不仅是一种回顾性分析，也是凝胶电泳在塑造作物蛋白质组学格局方面持久重要性的证明。
