采用高效液相色谱法对来自中国十一个省份的野葛花中两种异黄酮进行定量分析。

Quantitative analysis of two isoflavones in Pueraria lobata flowers from eleven Chinese provinces using high performance liquid chromatography.

机构信息

School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, Guangdong, PR China.

出版信息

Chin Med. 2010 Apr 23;5:14. doi: 10.1186/1749-8546-5-14.

DOI:10.1186/1749-8546-5-14

PMID:20416072

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2876171/

Abstract

BACKGROUND

Pueraria lobata flower (Gehua) is a medicinal herb to treat intoxication, hepatic and gastrointestinal tract lesion induced by alcohol. This study aims to develop a new HPLC method for the determination of two major isoflavones in P. lobata flowers, namely tectoridin and 6"-O-xylosyl-tectoridin.

METHODS

A high performance liquid chromatography (HPLC) method with a C18 column (250 mm x 4.6 mm, 5 mum) was developed for the quantitative analysis of tectoridin and 6"-O-xylosyl-tectoridin, the main isoflavone components in P. lobata flower. A simple gradient of acetonitrile/water (0 min 15:85; 35 min 50:50; 36 min 15:85; 40 min 15:85; v/v) was used, and 265 nm was selected as detection wavelength. Tectoridin and 6"-O-xylosyl-tectoridin were used as the external standards in quality control of P. lobata flower for the first time. The method was applied to practical use in quality assessment of eleven batches of P. lobata flower samples in Chinese herbal medicine market.

RESULTS

The peak area response was linear for tectoridin in the 11.8-236.4 mug/mL range with a correlation coefficient of 0.9996 (P < 0.001), and for 6"-O-xylosyl-tectoridin in the 10.33-185.99 mug/mL range with a correlation coefficient of 0.9984 (P < 0.001) respectively. The average recoveries were 102.7-103.7% for tectoridin and 95.7-103.2% for 6"-O-xylosyl-tectoridin (RSDs < 3%), and the intra-day and inter-day RSDs of the two components were less than 2%. This HPLC method was applied to assess the quality of P. lobata flower from eleven provinces in China. P. lobata flowers from northern China contained 26.46-43.28 mg/g of tectoridin and 30.90-48.23 mg/g of 6"-O-xylosyl-tectoridin comparing to 10.00-19.81 mg/g of tectoridin and 11.08-37.03 mg/g of 6"-O-xylosyl-tectoridin in those from southern China.

CONCLUSION

The results showed that P. lobata flowers from northern China contained more tectoridin and 6"-O-xylosyl-tectoridin than those from southern China.

摘要

背景

葛花是一种草药，用于治疗酒精引起的中毒和肝、胃肠道损伤。本研究旨在建立一种新的高效液相色谱法（HPLC），用于测定葛花中两种主要异黄酮，即大豆苷元和 6"-O-木糖基大豆苷元的含量。

方法

采用 C18 柱（250mm×4.6mm，5μm），建立了一种简单的梯度洗脱程序，用于定量分析葛花中的大豆苷元和 6"-O-木糖基大豆苷元。检测波长为 265nm。首次以大豆苷元和 6"-O-木糖基大豆苷元为对照品，对 11 批市售葛花样品进行质量控制。

结果

大豆苷元在 11.8-236.4μg/ml 范围内呈良好的线性关系，相关系数为 0.9996（P<0.001），6"-O-木糖基大豆苷元在 10.33-185.99μg/ml 范围内呈良好的线性关系，相关系数为 0.9984（P<0.001）。大豆苷元的平均回收率为 102.7-103.7%，6"-O-木糖基大豆苷元的平均回收率为 95.7-103.2%（RSDs<3%），日内和日间 RSD 均小于 2%。该 HPLC 法用于评价中国 11 个省的葛花质量。与来自中国南方的葛花（大豆苷元含量为 10.00-19.81μg/g，6"-O-木糖基大豆苷元含量为 11.08-37.03μg/g）相比，来自中国北方的葛花（大豆苷元含量为 26.46-43.28μg/g，6"-O-木糖基大豆苷元含量为 30.90-48.23μg/g）中含有更多的大豆苷元和 6"-O-木糖基大豆苷元。