Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon, 200-701, Korea.
Arch Pharm Res. 2010 Apr;33(4):619-23. doi: 10.1007/s12272-010-0417-9. Epub 2010 Apr 27.
Simultaneous determination method of four marker components, paeoniflorin, loganin, 5-hydroxymethyl-2-furaldehyde and paeonol in traditional herbal medicine, Yukmijihwang tang, was developed to achieve rapidly and systematic quality control by high performance liquid chromatography coupled with diode array detection. To develop this high performance liquid chromatography method, C(18) column (5 microm, 120 A, 4.6 mm x 150 mm) was used with gradient elution of water and methanol as mobile phase. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curves of standard components showed excellent linearity with R(2) > 0.9990. Limits of detection and limits of quantification were in the ranges 0.01-0.02 and 0.04-0.07 microg/mL, respectively. The relative standard deviations of data of the intra-day and inter-day experiments were less than 1.15% and 3.76%, respectively. The results of recovery test were found to range from 94.88 to 107.43% with relative standard deviation values 0.07-2.66%. The results of validation suggested that this method was very accurate and stabilized.
一种同时测定传统草药御乘载汤中 4 种标记成分（芍药苷、马钱苷酸、5-羟甲基糠醛和丹皮酚）的高效液相色谱二极管阵列检测法被开发出来，以便通过高效液相色谱快速而系统地进行质量控制。为了建立这种高效液相色谱法，采用 C（18）柱（5μm，120Å，4.6mm×150mm），以水和甲醇为流动相进行梯度洗脱。通过线性、回收率和精密度试验对色谱方法进行了验证。标准成分的校准曲线具有极好的线性，R（2）>0.9990。检测限和定量限分别在 0.01-0.02 和 0.04-0.07μg/mL 范围内。日内和日间实验数据的相对标准偏差均小于 1.15%和 3.76%。回收率试验的结果发现范围在 94.88%至 107.43%之间，相对标准偏差值为 0.07-2.66%。验证结果表明，该方法非常准确和稳定。
