Infertility and IVF Unit, Hospital for Women, Rabin Medical Center, Petach Tikva 49100, Israel.
Reprod Biomed Online. 2012 Oct;25(4):396-401. doi: 10.1016/j.rbmo.2012.07.002. Epub 2012 Jul 20.
The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks and from 35 girls and women aged 5-39 years. The specimens were prepared for detection of the FGF-10 protein by immunohistochemistry. Reverse-transcription PCR was applied to ovarian extracts to identify FGF-10 mRNA transcripts. In fetal tissue, the FGF-10 protein was detected in oocytes in 50% of the samples and in granulosa cells in 30%. In ovarian tissue from girls and women, the FGF-10 protein was detected in oocytes and granulosa cells in all samples. FGF-10 mRNA transcripts were present in all adult and fetal samples tested. The identification of FGF-10 at both the protein and mRNA levels suggests that FGF-10 may contribute to human preantral follicle development.
成纤维细胞生长因子 10（FGF-10）在人卵巢皮质卵泡中的表达尚未研究。本研究旨在探讨 FGF-10 在胎儿、女孩和妇女的原始卵泡中的表达。从 14 名妊娠 21-33 周的胎儿和 35 名 5-39 岁的女孩和妇女中获得卵巢样本。通过免疫组织化学检测卵巢标本中 FGF-10 蛋白的表达。应用逆转录 PCR 鉴定卵巢提取物中的 FGF-10 mRNA 转录本。在胎儿组织中，50%的样本中检测到 FGF-10 蛋白存在于卵母细胞中，30%的样本中存在于颗粒细胞中。在女孩和妇女的卵巢组织中，所有样本均检测到 FGF-10 蛋白存在于卵母细胞和颗粒细胞中。所有检测的成年和胎儿样本均存在 FGF-10 mRNA 转录本。FGF-10 在蛋白质和 mRNA 水平的鉴定表明，FGF-10 可能有助于人原始卵泡的发育。
