扫描隧道显微镜能对DNA进行测序吗？

Can the scanning tunneling microscope sequence DNA?

作者信息

Lindsay S M, Philipp M

机构信息

Department of Physics, Arizona State University, Tempe 85287-1504.

出版信息

Genet Anal Tech Appl. 1991 Feb;8(1):8-13. doi: 10.1016/1050-3862(91)90003-a.

DOI:10.1016/1050-3862(91)90003-a

PMID:2043383

Abstract

A revolutionary new microscope, the scanning tunneling microscope (STM), can image some surfaces at atomic resolution, even in air or water. It can produce high-resolution images of DNA, and we outline what we know of its mechanism, concluding that it may be able to sequence DNA. This application would require major advances in sample preparation in order for the technique to compete with conventional methods. On the other hand, the STM may provide a very useful alternative to gels for probing sequence-directed structural features.

摘要

一种革命性的新型显微镜——扫描隧道显微镜（STM），即使在空气或水中，也能以原子分辨率对某些表面进行成像。它可以生成DNA的高分辨率图像，我们概述了我们所了解的其工作机制，得出结论认为它或许能够对DNA进行测序。为了使该技术能与传统方法竞争，这种应用将需要在样品制备方面取得重大进展。另一方面，对于探测序列导向的结构特征，STM可能会成为凝胶的一种非常有用的替代方法。