Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.
Wiley Interdiscip Rev Syst Biol Med. 2011 Nov-Dec;3(6):702-16. doi: 10.1002/wsbm.154. Epub 2011 Jul 15.
Systems biology is a quantitative approach for understanding a biological system at its global level through systematic perturbation and integrated analysis of all its components. Simultaneous acquisition of information data sets pertaining to the system components (e.g., genome, proteome) is essential to implement this approach. There are limitations to such an approach in measuring gene expression levels and accounting for all proteins in the system. The success of genomic studies is critically dependent on polymerase chain reaction (PCR) for its amplification, but PCR is very uneven in amplifying the samples, ineffective in scarce samples and unreliable in low copy number transcripts. On the other hand, lack of amplifying techniques for proteins critically limits their identification to only a small fraction of high concentration proteins. Atomic force microscopy (AFM), AFM cantilever sensors, and AFM force spectroscopy in particular, could address these issues directly. In this article, we reviewed and assessed their potential role in systems biology.
系统生物学是一种通过系统干扰和所有组件的综合分析来理解生物系统整体水平的定量方法。同时获取与系统组件（例如基因组、蛋白质组）相关的信息数据集对于实施这种方法是至关重要的。这种方法在测量基因表达水平和考虑系统中的所有蛋白质方面存在局限性。基因组研究的成功在很大程度上取决于聚合酶链反应（PCR）来进行扩增，但 PCR 在扩增样本时非常不均匀，在样本稀少时效果不佳，在低拷贝数转录本时不可靠。另一方面，缺乏用于蛋白质的扩增技术严重限制了仅对高浓度蛋白质的一小部分进行鉴定。原子力显微镜（AFM）、AFM 悬臂传感器，特别是 AFM 力谱技术，可以直接解决这些问题。在本文中，我们回顾和评估了它们在系统生物学中的潜在作用。
