Division of Epidemiology, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, 137-134, Republic of Korea.
J Microbiol. 2010 Apr;48(2):236-42. doi: 10.1007/s12275-010-9220-z. Epub 2010 May 1.
A total of 66 Shigella sonnei isolates from 1999 to 2008 in Seoul was analyzed for their antimicrobial resistance, carriage of integron, and the patterns of Pulsed-field gel electrophoresis (PFGE). A high level of antimicrobial resistance to streptomycin (100%), trimethoprim/sulfamethoxazole (95%), tetracycline (94%), nalidixic acid (65%), and ampicillin (41%) was observed among S. sonnei isolates. Fourteen profiles of antimicrobial resistance were identified with the most common resistance profile being nalidixic acid, streptomycin, tetracycline, and trimethoprim/sulfamethoxazole (35%). PCR and DNA sequencing analysis revealed the presence of class 2 integron in all isolates, and class 1 and 2 integrons in 7 isolates. The class 2 integron carried two types of gene cassettes. One cassette array was dfrI, sat2, and aadA1 (91%), and the other was dfr1 and sat1 (8%). dfrA12 and aadA2 gene cassette was found in one isolate containing class 1 integron. PFGE was carried out to examine the genetic relatedness among isolates. All isolates except for one showed similar PFGE patterns (similarity of 80.1%). These results suggest that the S. sonnei isolated during 1999-2008 in Seoul have similar lineages that have not undergone evolutionary changes with time.
对 1999 年至 2008 年首尔的 66 株宋内志贺菌进行了抗菌药物耐药性、整合子携带情况和脉冲场凝胶电泳（PFGE）模式分析。宋内志贺菌对链霉素（100%）、甲氧苄啶-磺胺甲恶唑（95%）、四环素（94%）、萘啶酸（65%）和氨苄西林（41%）的耐药率较高。共鉴定出 14 种耐药谱，最常见的耐药谱为萘啶酸、链霉素、四环素和甲氧苄啶-磺胺甲恶唑（35%）。PCR 和 DNA 测序分析显示，所有分离株均携带 2 类整合子，7 株分离株携带 1 类和 2 类整合子。2 类整合子携带两种类型的基因盒。一种盒阵列为 dfrI、sat2 和 aadA1（91%），另一种为 dfr1 和 sat1（8%）。含有 1 类整合子的一个分离株中发现了 dfrA12 和 aadA2 基因盒。进行 PFGE 以检查分离株之间的遗传相关性。除 1 株外，所有分离株的 PFGE 图谱均相似（相似度为 80.1%）。这些结果表明，1999 年至 2008 年首尔分离的宋内志贺菌具有相似的谱系，随着时间的推移没有发生进化变化。
