Department of Pharmaceutical Chemistry, School of Pharmacy, University of Jena, Philosophenweg 14, D-07743 Jena, Germany.
J Pharm Biomed Anal. 2010 Dec 15;53(5):1076-90. doi: 10.1016/j.jpba.2010.04.005. Epub 2010 Apr 13.
In recent years, capillary electrophoresis (CE) has become a frequently used tool for enzyme assays due to its well-recognized advantages such as high separation efficiency, short analysis time, small sample and chemicals consumption. The published applications cover all aspects of enzyme characterization and analysis including the determination of the enzyme activity, substrate and modulator characterization and identification, as well as the investigation of enzyme-mediated metabolic pathways of bioactive molecules. The CE assays may be classified into two general categories: (1) pre-capillary assays where the reactions are performed offline followed by CE analysis of the substrates and products and (2) online assays when the enzyme reaction and separation of the analytes are performed in the same capillary. In online assays, the enzyme may be either immobilized or in solution. The latter is also referred to as electrophoretically mediated microanalysis (EMMA). The present review will highlight the literature of CE-based enzyme assays from 2006 to November 2009. One section will be devoted to applications of microfluidic devices.
近年来，毛细管电泳（CE）因其高分离效率、短分析时间、小样品和试剂消耗等公认的优点，已成为酶分析中常用的工具。已发表的应用涵盖了酶特性和分析的各个方面，包括酶活性的测定、底物和调节剂的特性和鉴定，以及对生物活性分子的酶介导代谢途径的研究。CE 测定法可分为两类：（1）毛细管前测定法，其中反应在线下进行，然后对底物和产物进行 CE 分析；（2）在线测定法，其中酶反应和分析物的分离在同一毛细管中进行。在在线测定中，酶可以固定化或溶解在溶液中。后者也称为电泳介导的微分析（EMMA）。本综述将重点介绍 2006 年至 2009 年 11 月期间基于 CE 的酶测定法的文献。其中一部分将专门介绍微流控器件的应用。
