Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, Leuven, Belgium; School of Medicine, University of California, San Diego, CA, USA.
Electrophoresis. 2014 Jan;35(1):119-27. doi: 10.1002/elps.201300294. Epub 2013 Oct 30.
This review gives an overview of the recent developments and applications in the use of CE-mediated microanalysis for enzyme studies. The period covers mid-2011 until mid-2013. Both off-line and in-line enzyme assays with their applications using CE are described in this article. For the in-capillary enzyme reaction, the techniques using electrophoretically mediated microanalysis (EMMA) as well as immobilized enzyme reactor (IMER) are discussed. The applications include the evaluation of enzyme activity, enzyme kinetics, enzyme inhibition, screening of enzyme inhibitors, and the study of enzyme-mediated drug metabolism.
本综述概述了近年来 CE 介导的微分析在酶研究中的最新发展和应用。该时期涵盖了 2011 年年中至 2013 年年中。本文描述了使用 CE 的离线和在线酶分析及其应用。对于毛细管内酶反应，讨论了使用电泳介导的微分析（EMMA）和固定化酶反应器（IMER）的技术。应用包括评估酶活性、酶动力学、酶抑制、酶抑制剂筛选以及酶介导的药物代谢研究。
