Patil S A, Tyagi P, Katoch K, Sengupta U
Department of Immunology, Central JALMA Institute for Leprosy, Uttar Pradesh, India.
Clin Exp Immunol. 1991 Jun;84(3):515-21.
Mycobacterium leprae antigens could be detected in the cerebrospinal fluid (CSF) of patients with leprosy, using a monoclonal-antibody-based sandwich immunoradiometric assay (SIRMA). Antigens of 12 kD, 35 kD and 30-40 kD were detected using ML06, ML04, and ML34 monoclonal antibodies, respectively. The 30-40-kD polysaccharide antigen, although present in larger amounts in M. leprae than the 12-kD and 35-kD protein antigens, was found in the CSF of comparatively fewer subjects. The antigen capture assay has been found sensitive to the level of nanograms. Avidin-biotin-based immunoblotting using pooled leprosy sera detected a larger number of antigens than using anti-M. leprae antisera raised in rabbits. The immunoblotting of CSF samples revealed about three antigens in the region of 100-160 kD and three more in the region of 45-60 kD as probed by leprosy sera. This study has for the first time revealed the presence of M. leprae antigens in the CSF of leprosy patients and the probable involvement of the central nervous system in leprosy.
使用基于单克隆抗体的夹心免疫放射分析（SIRMA），可在麻风病人的脑脊液（CSF）中检测到麻风分枝杆菌抗原。分别使用ML06、ML04和ML34单克隆抗体检测到了12kD、35kD和30 - 40kD的抗原。30 - 40kD的多糖抗原虽然在麻风分枝杆菌中的含量比12kD和35kD的蛋白质抗原多，但在相对较少的受试者脑脊液中被发现。已发现抗原捕获测定法对纳克水平敏感。使用麻风病人混合血清的抗生物素蛋白 - 生物素免疫印迹法检测到的抗原数量比使用兔抗麻风分枝杆菌抗血清检测到的更多。用麻风病人血清检测时，脑脊液样本的免疫印迹显示在100 - 160kD区域约有三种抗原，在45 - 60kD区域还有另外三种抗原。本研究首次揭示了麻风病人脑脊液中存在麻风分枝杆菌抗原以及中枢神经系统可能参与麻风病。
