Jaulneau Valérie, Lafitte Claude, Jacquet Christophe, Fournier Sylvie, Salamagne Sylvie, Briand Xavier, Esquerré-Tugayé Marie-Thérèse, Dumas Bernard
Laboratoire des Surfaces Cellulaires et Signalisation chez les Végétaux, Université de Toulouse, UPS, 24 Chemin de Borde Rouge, BP42617, Auzeville 31326 Castanet-Tolosan, France.
J Biomed Biotechnol. 2010;2010:525291. doi: 10.1155/2010/525291. Epub 2010 Apr 28.
The industrial use of elicitors as alternative tools for disease control needs the identification of abundant sources of them. We report on an elicitor obtained from the green algae Ulva spp. A fraction containing most exclusively the sulfated polysaccharide known as ulvan-induced expression of a GUS gene placed under the control of a lipoxygenase gene promoter. Gene expression profiling was performed upon ulvan treatments on Medicago truncatula and compared to phytohormone effects. Ulvan induced a gene expression signature similar to that observed upon methyl jasmonate treatment (MeJA). Involvement of jasmonic acid (JA) in ulvan response was confirmed by detecting induction of protease inhibitory activity and by hormonal profiling of JA, salicylic acid (SA) and abscisic acid (ABA). Ulvan activity on the hormonal pathway was further consolidated by using Arabidopsis hormonal mutants. Altogether, our results demonstrate that green algae are a potential reservoir of ulvan elicitor which acts through the JA pathway.
将激发子作为疾病控制的替代工具进行工业应用，需要确定其丰富的来源。我们报道了一种从绿藻石莼属中获得的激发子。一个几乎只含有被称为石莼聚糖的硫酸化多糖的组分，能诱导受脂氧合酶基因启动子控制的GUS基因表达。在用石莼聚糖处理蒺藜苜蓿后进行了基因表达谱分析，并与植物激素的作用进行了比较。石莼聚糖诱导的基因表达特征与茉莉酸甲酯（MeJA）处理后观察到的相似。通过检测蛋白酶抑制活性的诱导以及对茉莉酸（JA）、水杨酸（SA）和脱落酸（ABA）进行激素谱分析，证实了JA参与石莼聚糖反应。通过使用拟南芥激素突变体，进一步巩固了石莼聚糖在激素途径上的活性。总之，我们的结果表明绿藻是石莼聚糖激发子的潜在储存库，其通过JA途径发挥作用。
