Watanabe T, Pratt R M
Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, Japan.
Experientia. 1991 May 15;47(5):493-7. doi: 10.1007/BF01959953.
The effects of all-trans-retinoic acid (RA) (tretinoin) on the craniofacial development of mouse embryos were examined using whole embryo culture. In day 8 embryos cultured for 48 h, embryonic growth was inhibited concentration-dependently by all-trans-RA treatment. Most of the treated embryos exhibited hypoplasia of the primary palatal processes and a reduction in the development of the first visceral arches. In day 10 embryos cultured for 48 h, although embryonic growth was not inhibited at any concentrations of all-trans-RA, median cleft lip (93%), hypoplasia of the primary palatal processes (37%) and limb reduction deformities (48%) occurred commonly. Furthermore, RA treatment greatly reduced the size of the secondary palatal processes. The incorporation of 3H-thymidine in the treated maxillary processes was decreased to 65% of the control value at 1.0 x 10(-7) M all-trans-RA. These findings indicate that all-trans-RA is teratogenic in mouse whole embryo culture.
使用全胚胎培养法研究了全反式维甲酸（RA）（维甲酸）对小鼠胚胎颅面发育的影响。在培养48小时的第8天胚胎中，全反式维甲酸处理浓度依赖性地抑制胚胎生长。大多数经处理的胚胎表现出原发腭突发育不全以及第一鳃弓发育减少。在培养48小时的第10天胚胎中，尽管在任何浓度的全反式维甲酸下胚胎生长均未受到抑制，但正中唇裂（93%）、原发腭突发育不全（37%）和肢体减少畸形（48%）普遍发生。此外，维甲酸处理极大地减小了继发腭突的大小。在1.0×10⁻⁷ M全反式维甲酸作用下，处理后的上颌突中³H-胸腺嘧啶核苷的掺入量降至对照值的65%。这些发现表明全反式维甲酸在小鼠全胚胎培养中具有致畸性。
