植物病原真菌禾谷镰刀菌的 2C 型蛋白磷酸酶 FgPtc1p 参与锂毒性和毒力。
The type 2C protein phosphatase FgPtc1p of the plant fungal pathogen Fusarium graminearum is involved in lithium toxicity and virulence.
机构信息
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
出版信息
Mol Plant Pathol. 2010 Mar;11(2):277-82. doi: 10.1111/j.1364-3703.2009.00598.x.
Abstract
Type 2C protein phosphatases (PP2Cs) are monomeric protein serine/threonine phosphatases that play various roles in eukaryotic organisms. In this study, we characterized the PP2C encoded by FgPTC1 in Fusarium graminearum, the major causal agent of Fusarium head blight on wheat and barley. We found that deletion of FgPTC1 delays the mycelium growth of F. graminearum in response to lithium. Consistently, FgPTC1 complemented the function of ScPTC1 in lithium toxicity in Saccharomyces cerevisiae. Furthermore, we showed that deletion of FgPTC1 attenuated the virulence of F. graminearum on wheat. Therefore, FgPTC1 plays an important role in regulating the hyphal growth and virulence of F. graminearum.
摘要
2C 型蛋白磷酸酶（PP2Cs）是单体蛋白丝氨酸/苏氨酸磷酸酶，在真核生物中发挥多种作用。在本研究中，我们对禾谷镰孢中由 FgPTC1 编码的 PP2C 进行了表征，禾谷镰孢是小麦和大麦赤霉病的主要病原体。我们发现，FgPTC1 的缺失会延迟禾谷镰孢菌丝对锂的生长响应。一致地，FgPTC1 互补了酿酒酵母中 ScPTC1 在锂毒性中的功能。此外，我们表明，FgPTC1 的缺失削弱了禾谷镰孢对小麦的毒性。因此，FgPTC1 在调节禾谷镰孢菌丝生长和毒性方面发挥着重要作用。
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