Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Ecole Nationale Vétérinaire de Nantes (ENVN), USC INRA 2013, BP 50707, F-44307 Nantes Cedex 3, France.
Analyst. 2009 Aug;134(8):1637-46. doi: 10.1039/b901813a. Epub 2009 May 6.
Beta-agonist compounds can be misused in food-producing animals for growth promoting purposes. Efficient methods based on mass spectrometry detection have been developed to ensure the control of such veterinary drug residues. Nevertheless, the use of "cocktails" composed of mixtures of low amounts of several substances as well as the synthesis of new compounds of unknown structure prevent efficient prevention. To circumvent those problems, new analytical tools able to detect such abuse are today mandatory. In this context, metabolomics may represent a new emerging strategy for investigating the global physiological effects associated to a family of substances and therefore, to suspect the administration of beta-agonists (either "cocktails" or unknown compounds). As a first demonstration of feasibility, an untargeted metabolomic approach based on liquid chromatography coupled to high resolution mass spectrometry measurements was developed and made it possible to highlight metabolic modifications in urine consecutively to a clenbuterol administration. By the means of chemometrics, those metabolic differences were used to build predictive models able to suspect clenbuterol administration in calves. This new approach may be considered of valuable interest to overcome current limitations in the control of growth promoters' abuse, with promising perspectives in terms of screening.
β-激动剂化合物可被滥用于生长促进目的的食用动物。已经开发了基于质谱检测的高效方法来确保此类兽药残留的控制。然而，使用由几种物质的低量混合物组成的“混合物”以及合成未知结构的新化合物会妨碍有效的预防。为了规避这些问题，今天必须使用能够检测此类滥用的新分析工具。在这种情况下，代谢组学可能代表了一种新的新兴策略，用于研究与一类物质相关的整体生理效应，因此，可以怀疑β-激动剂（无论是“混合物”还是未知化合物）的给药。作为可行性的第一个演示，开发了一种基于液相色谱与高分辨率质谱联用的非靶向代谢组学方法，该方法能够突出在克伦特罗给药后尿液中的代谢变化。通过化学计量学手段，这些代谢差异被用于构建能够怀疑小牛中克伦特罗给药的预测模型。这种新方法可能被认为具有克服当前生长促进剂滥用控制中的局限性的重要意义，在筛选方面具有广阔的前景。
