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中空金-银纳米粒子标记免疫层析条用于克伦特罗的高灵敏检测。

Hollow Au-Ag Nanoparticles Labeled Immunochromatography Strip for Highly Sensitive Detection of Clenbuterol.

机构信息

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

Division of Polymer and Composite Materials, Ningbo Institute of Material Technology and Engineering Chinese Academy of Sciences, No. 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.

出版信息

Sci Rep. 2017 Jan 30;7:41419. doi: 10.1038/srep41419.

DOI:10.1038/srep41419
PMID:28134263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5278391/
Abstract

The probe materials play a significant role in improving the detection efficiency and sensitivity of lateral-flow immunochromatographic test strip (ICTS). Unlike conventional ICTS assay usually uses single-component, solid gold nanoparticles as labeled probes, in our present study, a bimetallic, hollow Au-Ag nanoparticles (NPs) labeled ICTS was successfully developed for the detection of clenbuterol (CLE). The hollow Au-Ag NPs with different Au/Ag mole ratio and tunable size were synthesized by varying the volume ratio of [HAuCl4]:[Ag NPs] via the galvanic replacement reaction. The surface of hollow Ag-Au NPs was functionalized with 11-mercaptoundecanoic acid (MUA) for further covalently bonded with anti-CLE monoclonal antibody. Overall size of the Au-Ag NPs, size of the holes within individual NPs and also Au/Ag mole ratio have been systematically optimized to amplify both the visual inspection signals and the quantitative data. The sensitivity of optimized hollow Au-Ag NPs probes has been achieved even as low as 2 ppb in a short time (within 15 min), which is superior over the detection performance of conventional test strip using Au NPs. The optimized hollow Au-Ag NPs labeled test strip can be used as an ideal candidate for the rapid screening of CLE in food samples.

摘要

探针材料在提高侧向流动免疫层析测试条（ICTS）的检测效率和灵敏度方面起着重要作用。与传统的 ICTS 分析通常使用单一组分、固态金纳米粒子作为标记探针不同，在本研究中，成功开发了一种双金属、中空 Au-Ag 纳米粒子（NPs）标记 ICTS，用于检测克伦特罗（CLE）。通过改变[HAuCl4]：[Ag NPs]的体积比，通过电置换反应合成了具有不同 Au/Ag 摩尔比和可调尺寸的中空 Au-Ag NPs。中空 Ag-Au NPs 的表面用 11-巯基十一酸（MUA）进行功能化，以便进一步与抗 CLE 单克隆抗体共价结合。Au-Ag NPs 的整体尺寸、单个 NPs 内的孔的尺寸以及 Au/Ag 摩尔比已被系统优化，以放大目视检查信号和定量数据。优化后的中空 Au-Ag NPs 探针的灵敏度甚至可以在短时间内（15 分钟内）低至 2 ppb，优于使用 Au NPs 的传统测试条的检测性能。优化后的中空 Au-Ag NPs 标记测试条可作为快速筛选食品样品中 CLE 的理想候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/aa163255f090/srep41419-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/4fa82c22a18d/srep41419-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/cf37409196e2/srep41419-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/203a43b877f1/srep41419-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/44854b21d972/srep41419-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/aae8b4977e0a/srep41419-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/3608585d0cab/srep41419-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/aa163255f090/srep41419-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/4fa82c22a18d/srep41419-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/cf37409196e2/srep41419-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/203a43b877f1/srep41419-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/44854b21d972/srep41419-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/aae8b4977e0a/srep41419-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/3608585d0cab/srep41419-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8187/5278391/aa163255f090/srep41419-f7.jpg

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