Chemistry Department, College of Sciences, Shiraz University, Shiraz, 7145685464, Iran.
Analyst. 2009 Aug;134(8):1692-8. doi: 10.1039/b901273g. Epub 2009 Jun 9.
In this study, a new, simple, fast and inexpensive method as an alternative to visible spectrophotometry is developed. In this method the cells containing the sample solution were scanned with a scanner, then the color of each cell was analyzed with software written in visual basic (VB 6) media to red, green and blue values. The cells were built by creating holes in the Plexiglas sheet. The dimensions of identical cells were examined by Cr (III) solution with known concentrations. The validity of this new method was studied by determination of dopamine (DA) without using any other reagent. The parameters which affect the system were optimized. The comparison between the current and traditional UV-Vis spectrophotometry methods was studied and the results revealed similar trends in both methods. The developed method was successfully applied to the determination of dopamine in serum and urine without using any pretreatment. Finally comparing the results obtained in the developed method showed that microwave irradiation of the solution can decrease the experimental time, increase sensitivity and improve the limit of detection.
在这项研究中，开发了一种新的、简单、快速且廉价的方法，以替代可见分光光度法。在该方法中，用扫描仪扫描含有样品溶液的细胞，然后用 VB6 编写的软件分析每个细胞的颜色，得出红、绿、蓝值。细胞是通过在有机玻璃片上打孔来制作的。通过用已知浓度的 Cr(III)溶液检查相同细胞的尺寸。通过不使用任何其他试剂测定多巴胺（DA）来研究这种新方法的有效性。优化了影响该系统的参数。研究了当前方法和传统的 UV-Vis 分光光度法之间的比较，结果表明两种方法具有相似的趋势。该方法成功地应用于血清和尿液中多巴胺的测定，无需任何预处理。最后，对开发方法得到的结果进行比较，表明溶液的微波辐射可以缩短实验时间，提高灵敏度并改善检测限。
