The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201210, China.
Phytochemistry. 2010 Jul;71(10):1174-80. doi: 10.1016/j.phytochem.2010.04.002. Epub 2010 May 7.
Three norclerodane diterpenoids, diosbulbins K-M, and one analogous enolglycoside, diosbulbinoside G, together with four norclerodane diterpenoids, diosbulbins B, E, F and G, were isolated from rhizomes of Dioscorea bulbifera. Their structures were established by spectroscopic and chemical methods, including 1H and 13C NMR, NOESY, HSQC, HMBC, and HRMS analyses. The relative configurations of diosbulbins K and L, and diosbulbin F were confirmed by X-ray crystallographic diffraction analysis, and the absolute stereochemistry of diosbulbin K was determined by a modified Mosher's method. The 13C NMR spectroscopic data for diosbulbins E, F and G were also measured for the first time. The compounds did not show significant cytotoxic and anti-bacterial activities.
从盾叶薯蓣块茎中分离得到了三种新的诺卡烷二萜类化合物（diosbulbins K-M）和一种类似的烯醇糖苷类化合物（diosbulbinoside G），以及四种诺卡烷二萜类化合物（diosbulbins B、E、F 和 G）。通过光谱和化学方法，包括 1H 和 13C NMR、NOESY、HSQC、HMBC 和 HRMS 分析，确定了它们的结构。通过 X 射线晶体衍射分析确定了 diosbulbins K 和 L 以及 diosbulbin F 的相对构型，并通过改良的 Mosher 法确定了 diosbulbin K 的绝对立体化学构型。此外，还首次测量了 diosbulbins E、F 和 G 的 13C NMR 谱数据。这些化合物没有表现出显著的细胞毒性和抗菌活性。
