Pinheiro Céline, Reis Rui M, Ricardo Sara, Longatto-Filho Adhemar, Schmitt Fernando, Baltazar Fátima
Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal.
J Biomed Biotechnol. 2010;2010:427694. doi: 10.1155/2010/427694. Epub 2010 May 4.
Monocarboxylate transporters (MCTs) are important cellular pH regulators in cancer cells; however, the value of MCT expression in cancer is still poorly understood. In the present study, we analysed MCT1, MCT2, and MCT4 protein expression in breast, colon, lung, and ovary neoplasms, as well as CD147 and CD44. MCT expression frequency was high and heterogeneous among the different tumours. Comparing with normal tissues, there was an increase in MCT1 and MCT4 expressions in breast carcinoma and a decrease in MCT4 plasma membrane expression in lung cancer. There were associations between CD147 and MCT1 expressions in ovarian cancer as well as between CD147 and MCT4 in both breast and lung cancers. CD44 was only associated with MCT1 plasma membrane expression in lung cancer. An important number of MCT1 positive cases are negative for both chaperones, suggesting that MCT plasma membrane expression in tumours may depend on a yet nonidentified regulatory protein.
单羧酸转运蛋白（MCTs）是癌细胞中重要的细胞pH调节剂；然而，MCT表达在癌症中的价值仍知之甚少。在本研究中，我们分析了乳腺癌、结肠癌、肺癌和卵巢肿瘤中MCT1、MCT2和MCT4蛋白的表达，以及CD147和CD44的表达。MCT表达频率在不同肿瘤中较高且存在异质性。与正常组织相比，乳腺癌中MCT1和MCT4表达增加，肺癌中MCT4质膜表达降低。卵巢癌中CD147与MCT1表达之间以及乳腺癌和肺癌中CD147与MCT4之间存在关联。CD44仅与肺癌中MCT1质膜表达相关。大量MCT1阳性病例的两种伴侣蛋白均为阴性，这表明肿瘤中MCT质膜表达可能依赖于一种尚未确定的调节蛋白。
