JEG-3 滋养层样细胞来源的麦芽寡糖具有免疫调节特性。
Maltooligosaccharides from JEG-3 trophoblast-like cells exhibit immunoregulatory properties.
机构信息
Departments of Ophthalmology and Visual Sciences and Microbiology and Immunology, The University of Michigan Medical School, Ann Arbor, MI 48105, USA.
出版信息
Am J Reprod Immunol. 2011 Jan;65(1):54-64. doi: 10.1111/j.1600-0897.2010.00851.x.
PROBLEM
to better understand the immunoregulatory properties of trophoblasts, we have searched for small immunologically active carbohydrates derived from intact trophoblast-like cells.
METHOD OF STUDY
using solid phase extraction coupled with HPLC and mass spectrometry methods, we have characterized a low molecular weight carbohydrate-rich fraction associated with JEG-3 cells. We have also tested the bioactivities of selected authentic oligosaccharides found in the oligosaccharide fraction.
RESULTS
the most abundant components of the low molecular weight carbohydrate-rich fraction were maltotriose and maltotetraose, with detectable amounts of maltopentaose. When authentic maltooligosaccharides were tested using lymphocytes, IL-2 inhibition was observed. This activity was dependent upon the number of saccharide subunits, stereochemistry, and concentration. To further test maltooligosaccharide properties, maltopentose was attached to glass cover slips. Although spontaneous neutrophil motility was observed on unmodified and control surfaces, it was inhibited on maltooligosaccharide-derivatized surfaces.
CONCLUSION
maltooligosaccharides are associated with the trophoblast's surface where they may exhibit immunoregulatory activities.
问题
为了更好地了解滋养层的免疫调节特性，我们已经在寻找来自完整滋养层样细胞的具有免疫活性的小碳水化合物。
方法
使用固相萃取与 HPLC 和质谱方法相结合，我们对与 JEG-3 细胞相关的低分子量富含碳水化合物的部分进行了表征。我们还测试了在寡糖部分中发现的选定天然寡糖的生物活性。
结果
低分子量富含碳水化合物部分的最丰富成分是麦芽三糖和麦芽四糖，麦芽五糖也有可检测到的量。当使用淋巴细胞测试天然麦芽寡糖时，观察到 IL-2 抑制。这种活性取决于糖单位的数量、立体化学和浓度。为了进一步测试麦芽寡糖的性质，将麦芽五糖连接到玻璃载玻片上。尽管在未修饰和对照表面上观察到自发的中性粒细胞运动，但在麦芽寡糖衍生的表面上则受到抑制。
结论
麦芽寡糖与滋养层表面相关，在那里它们可能表现出免疫调节活性。
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